摘要
目的采用实时荧光聚合酶链反应(PCR)法检测手足口病患儿病原体。方法收集433例疑似手足口病患儿的粪便标本进行肠道病毒通用型、肠道病毒71型(EV-71)和柯萨奇病毒16型(CA-16)鉴别与分析。结果实时荧光PCR法检测433例患儿肛拭子标本使用通用型试剂盒检出366例阳性,其中66例为EV-71感染,151例为CA-16感染。结论 EV-71和CA-16肠道病毒是手足口病的主要病原体,该地区2011年以CA-16感染为主,实时荧光PCR技术对手足口病的病原诊断具有重要价值。
Objective To detect the pathogens of hand,foot and mouth disease（HFMD） by real-time fluores cence PCR. Methods The fecal samples in 433 children patients with suspected HFMD were collected for the identi- fication and analysis of universal intestinal virus,EV-71 and CoxA-16. Results Among 433 rectal swab specimens de- tected by real-time fluorescence PCR, 366 specimens were positive,in which, 151 specimens were CA-16 infection and 66 specimens were EV-71 infection. Conclusion CoxA-16 and EV 71 viruses are the main pathogens in HFMD. Cox A-16 virus is the main pathogen of HFMD in the native area this year. Real-time fluorescence PCR technique has im- portant value in the pathogen diagnosis of HFMD.
出处
《检验医学与临床》
CAS
2013年第5期522-523,共2页
Laboratory Medicine and Clinic
