Kindlin-2在胃癌组织中的表达及其与肿瘤侵袭转移的关系

Clinical significance of Kindlin-2 protein in gastric cancer

目的探讨胃癌组织中Kindlin-2的表达及其与肿瘤侵袭转移和预后的关系。方法连续收集50例胃癌患者手术切除的胃癌及相应癌旁组织标本，应用蛋白印迹进法及定时定量聚合酶链反应检测Kindlin-2在蛋白水平及RNA水平的表达，并分析其与胃癌患者临床病理指标及预后的相关性。结果Kindlin-2在胃癌组织中的表达水平明显高于癌旁组织[（0．421±0．334）比（0．137±0．016）]，差异有统计学意义（P=0．012）。胃癌组织Kindlin-2／[3-actinmRNA的表达水平明显高于癌旁组织[（1．05±0．20）比（0．77±0．14）]，差异有统计学意义（P=0．034）。Kindlin-2蛋白表达水平与患者年龄、性别和肿瘤分化程度无明显关系。肿瘤侵犯深度为T3-4患者（12例）的Kindlin-2表达明显高于T1-2（38例）患者。N3、N3期患者（分别为14、13例）的Kindlin-2表达水平明显高于N，期患者（23例），差异有统计学意义（P=0．013）。TNM综合分期为Ⅲ-Ⅳ期的患者Kindlin-2表达水平明显高于I～Ⅱ期的患者（12例），差异有统计学意义（P=0．016）。Kindlin-2高表达胃癌患者（27例）平均生存时间明显短于低表达患者（23例）[（23-4-4）个月比（65-4-10）个月]，差异有统计学意义（P=0．017）。Kindlin-2高表达胃癌患者无病生存时间亦短于低表达患者[（12±4）个月比（55±10）个月]，差异有统计学意义（P=0．019）。结论Kindlin-2可能在胃癌发生发展中起重要作用，并且可能成为靶向治疗及评估胃癌患者预后的指标。

Objective To explore the relationship between Kindlin-2 expression in gastric cancer and tumor invasion, metastasis, and the outcome of patients. Methods Kindlin-2 expressions were detected by Western blot and real-time polymerase chain reaction （PCR） at protein and RNA levels in 50 pairs of gastric eaneer samples. The correlations between Kindlin-2 expression and clinicopathologic factors were analyzed. Results Kindlin-2 was up-regulated in gastric cancer tissues, （0.421 ±0.334）compared to（0. 137 ± 0. 016）. Kindlin-2 expression had a negative correlation with patients age, sex and tumor differentiation. Kindlin-2 expression was higher in T3 -4 patients compared to T1 -2. Kindlin-2 expression was higher in N2 -3 patients compared to N1. Kindlin-2 expression was higher in I - Ⅱpatients compared to Ⅲ-1V. Patients with high Kindlin-2 expression had significantly progression-free survival, （ 12±4） months compared to（55 ± 10） months, P =0. 019. Conclusion The expression of Kindlin-2 may play an important role in the development of gastric cancer and may be used to evaluate the prognosis of gastric cancer.