多药耐药基因1在肺癌中的表达及临床病理意义

Expression of MDR1 in lung cancer and its significance in clinical pathology

目的探讨多药耐药基因1(MDR1)在肺癌中的表达及对其生物学行为的影响。方法采用免疫组织化学法,对60例肺癌及癌旁正常组织中MDR1进行检测。结果 MDR1蛋白阳性产物主要定位于胞质和胞膜,呈棕黄色颗粒,少部分定位于胞核。癌旁正常组织及癌组织的表达二者比较,其差异有统计学意义(P<0.05);在不同组织学类型肺癌中的阳性表达率有所不同,非小细胞肺癌(NSCLC)与小细胞癌(SCLC)之间的比较差异有统计学意义(P<0.05);在不同分化程度、有无淋巴结转移及TNM分期组间比较差异无统计学意义(P>0.05)。结论耐药性的产生可能是发生在NSCLC的早期事件并随着病程的进展有加强趋势;SCLC对化疗药物相对敏感。

Objective To explore the expression of MDR1 in lung cancer and its biological behavior influ- ence. Methods Using immunohistochemistry by SP kit, the expressions of MDR1 in cancer and normal tissue adjacent to cancer were examined in 60 cases of lung eaneer. Results MDR1 protein positive products, whieh are tan-yellow particles, are mainly located in the eytoplasm and cell membrane, and some of them are positioned in the cell nucleus. Comparing the expressions of normal tissue adjacent to cancer with ones of cancer, the difference is statistically significant （P〈0.05）;In different histologic types of lung cancer, the positive expression rate is different, and the differenee between non-small cell lung cancer （squamous cell earcinoma, adenoearcinoma, large cell carcinoma） and small cell carcinoma is statistically significant （P〈0.05）. The relative differences among expressions in different differentiation degree, with or without lymph node metastasis and in TNM staging are not statistically significant （P〉0.05）. Conclusion Drug resistance may be an early event occurred in non-small eell lung cancer, and the trend is strengthened with the progress of the course; small cell lung cancer is relatively sensitive to chemotherapy drugs.