电针对大鼠局灶性脑缺血再灌注后脑内血管内皮生长因子受体-2 mRNA和脑源性神经营养因子蛋白表达的影响

Effect of Electroacupuncture on Expression of Vascular Endothelial Growth Factor Receptor-2 mRNA and Brain-Derived Neurotrophic Factor Protein in Brain Tissue of Rats with Focal Cerebral Ischemia/Reperfusion

目的观测电针大鼠双侧合谷穴(LI 4)对局灶性脑缺血再灌注后脑内血管内皮生长因子受体-2(VEGFR-2)mRNA和脑源性神经营养因子(BDNF)蛋白表达的影响,探讨神经发生和血管生成间可能的相互联系和作用。方法线栓法制备大鼠局灶性脑缺血再灌注模型。90只大鼠随机分为对照组(n=6)、模型组(n=42)和电针组(n=42)。观察局灶性脑缺血1 h后再灌注2 h、12 h、24h、3 d、7 d、14 d、21 d共7个时相点,每个时相点6只大鼠。采用反转录酶-聚合酶链锁反应(RT-PCR)法检测VEGFR-2 mRNA的表达,免疫组化法检测BDNF蛋白的表达。结果再灌注后12 h、24 h,电针组VEGFR-2 mRNA明显高于模型组(P<0.01),3 d也高于模型组(P<0.05)。与模型组比较,电针组海马区BDNF蛋白在再灌注后2 h开始增加(P<0.05),24 h达高峰(P<0.01),3 d后仍有显著性差异(P<0.05),但呈现增加幅度降低的趋势。结论电针能促进脑缺血再灌注后VEGFR-2 mRNA、BDNF蛋白的表达;神经血管因子可能是神经发生与血管生成联系与作用的分子学基础。

Objective To observe the effect of electroacupuncture on the expression of vascular endothelial growth factor receptor-2 （VEGFR-2） mRNA and brain-derived neurotrophic factor （BDNF） protein in brain tissue of rats with focal cerebral ischemia/reperfusion. Methods The focal cerebral ischemia/reperfusion model was established with filament occlusion. 90 rats were randomly divided into control group （n=6）, model group （n=42） and electroacupuncture group （n=42）. They were observed 2 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d after isch- emia/reperfusion, 6 rats in each points. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression of VEG- FR-2 mRNA. Immunohistology method was used to detect the expression of BDNF protein. Results The expression of VEGFR-2 mRNA was higher in the electroacupuncture group than in the model group 12 h, 24 h, 3 d after reperfusion （P〈0.05）. The expression of BDNF pro- tein in the hippocampus increased 2 h （P〈0.05）, reached peak 24 h （P〈0.01） and still higher 3 d （P〈0.05） in the electroacupuncture group than in the model group after reperfusion. Conclusion Electroacupuncture can upregulate the expression of VEGFR-2 mRNA and BDNF protein in brain tissue of rats with focal cerebral ischemia/reperfusion. Neuro-angiogenic factor is possibly the molecular basis of coordinat- ed interaction of the nervous and vascular system.