电针对局灶性脑缺血再灌注大鼠神经生长相关蛋白-43和Nogo-A表达的影响

Effects of Electroacupuncture on Expression of Growth Associated Protein-43 and Nogo-A after Focal Cerebral Ischemia/Reperfusion in Rats

目的探讨电针对局灶性脑缺血再灌注模型大鼠神经功能恢复和脑组织神经生长相关蛋白-43(GAP-43)、Nogo-A表达的影响。方法 48只健康成年雄性Sprague-Dawley大鼠分为假手术组、模型组和电针组,每组16只。采用线栓法制作大鼠大脑中动脉缺血再灌注模型。电针组于造模成功90 min后电针刺双侧内关、水沟、三阴交和百会30 min,每天1次。假手术组和模型组不进行任何干预治疗。各组大鼠在造模后7 d、14 d各取8只进行神经功能评估及免疫组化SP法观察脑组织GAP-43、Nogo-A的表达。结果假手术组大鼠无神经功能缺损症状;7 d时,模型组和电针组神经功能评分无显著性差异(P>0.05),14 d时,电针组神经功能优于模型组(P<0.05)。假手术组各时间点未见GAP-43阳性细胞表达。7 d时,模型组缺血灶周围脑组织出现GAP-43阳性细胞,14 d时减少;电针组GAP-43阳性细胞各时间点较模型组明显增加(P<0.01)。假手术组仅见少量Nogo-A表达,模型组脑缺血区7 d、14 d时Nogo-A表达明显增多(P<0.01),电针组Nogo-A表达在各时间点较模型组明显减少(P<0.01)。结论电针能通过促进局灶性脑梗死大鼠脑组织内GAP-43表达、抑制Nogo-A表达,改善大鼠的神经功能。

Objective To investigate the effect of electroacupuncture on expression of growth associated protein-43 （GAP-43） and No- go-A in brain after focal cerebral ischemia/reperfusion in rats. Methods 48 male Sprague-Dawley rats were randomly divided into sham group, model group and electroacupuncture group, and the latter two were modeled as middle cerebral artery occlusion and reperfusion with nylon monofilament suture. Electroacupuncture was performed 90 min after modeling in the electroacupucture group at acupoints of Nei- guan （PC06）, Shuigou （DU26）, Sanyinjiao （SP06）, Baihui （DU20）, for 30 min, once a day. The sham group and the model group were con- ventionally fed in cages without any intervention. 8 rats of each group were assessed with Longa＇s score, and the expressions of GAP-43 and Nogo-A were detected with immunohistochemistry 7 d and 14 d after modeling respectively. Results The sham group presented no neurolog- ical symptoms. There was not different in Longa＇s score between the model group and the electroacupuncture group 7 d after modeling （P〉 0.05）, but was different 14 d after modeling （P〈0.05）. GAP-43 positive cells was not found in the sham group, but could be found around ce- rebral ischemia 7 d after modeling, which decreased 14 d after modeling in the model group. GAP-43 positive cells increased significantly in the electroacupuncture group compared with the model group at each time （P〈0.01）. Nogo-A positive cells was few found in the sham group, and was more in the model group （P〈0.01）. The expression of Nogo-A decreased significantly in the electroacuptmcture group com- pared with the model group at each time （P〈0.01）. Conclusion Electroacupuncture can improve neurological function of focal cerebral isch- emia/reperfusion rats, which may be associated with the increase of GAP-43 and descrease of Nogo-A in peri-infarct regions.