番茄红素抗氧化应激效应对大鼠脊髓损伤后运动功能恢复的影响

Anti-oxidative Stress Effect of Lycopene on Motor Function after Spinal Cord Injury in Rats

目的观察番茄红素抗氧化应激效应对大鼠脊髓损伤后运动功能恢复的影响。方法健康成年Sprague-Dawley大鼠36只,采用Allen法(10 g×25 mm)在T9造成大鼠脊髓损伤模型,并随机分为对照组(A组)、甲泼尼龙琥珀酸钠(MP)治疗组(B组)和番茄红素治疗组(C组),每组12只。造模成功30 min后,A组不给予治疗,B组腹腔注射MP 30 mg/kg,C组灌胃番茄红素20 mg/kg。于术后1 d、3 d、7 d分别对各组进行斜板试验、后肢功能BBB评分、血清中的超氧化物歧化酶(SOD)和丙二醛(MDA)测定。结果与A组相比,C组1 d、3 d和B组1 d、3 d、7 d斜板试验评分升高(P<0.05);C组1 d、3 d、7 d和B组1 d、7 d BBB评分升高(P<0.05);C组1 d、3 d、7 d和B组3 d、7 d SOD活性升高(P<0.05);C组3 d、7 d和B组1 d、7 d MDA含量降低(P<0.05)。结论番茄红素可以降低急性脊髓损伤后的氧化应激水平,促进大鼠运动功能恢复。

Objective To observe the influence of anti-oxidative stress effect of lycopene （LP） on motor function after spinal cord injury （SCI） in rats. Methods 36 healthy adult Sprague-Dawley rats were randomly divided into Groups A, B and C with 12 rats in each group. SCI model was made by Allen＇s mode （10 gx25 mm） on Tg. Group A received no treatment, 30 mg/kg methylprednisolone sodium succinate was injected for Group B in peritoneal cavity 30 min after SCI, 20 mg/kg LP was given orally for Group C. On the 1st d, 3rd d and 7th d af- ter operation, the motor function was assessed by slanting board test and Basso-Beattie-Bresnahan scale （BBB）; superoxide dismutase （SOD） and malondialdehyde （MDA） were determined in serum. Results Compared with Group A, the scores of the slanting board test signif- icantly increased in Group C 1 d, 3 d, and in Group B 1 d, 3 d and 7 d after operation （P〈0.05）; the BBB scores significantly increased in Group C 1 d, 3 d and 7 d, and in Group B 1 d, 7 d after operation （P〈0.05）; the activity of SOD significantly increased in Group C 1 d, 3 d and 7 d, and in Group B 3 d, 7 d after operation （P〈0.05）; the content of MDA significantly decreased in Group C 3 d, 7 d, and in Group B 1 d, 7 d after operation （P〈0.05）. Conclusion Lycopene can reduce the level of oxidative stress and promote the motor function in rats after acute SCI.