蓝萼甲素的急性毒性实验研究

Acute Toxicity of Glaucocalyxin A

目的:观察蓝萼甲素对小鼠的急性毒性,评价其安全性,为新药研发和临床用药提供理论依据。方法:采用口服和腹腔注射给药途径,观察小鼠给予不同剂量蓝萼甲素后的死亡率,Bliss法计算半数致死量。结果:蓝萼甲素口服1次的MTD为2.0 g.kg-1.d-1,口服给药未能测出蓝萼甲素的LD50,蓝萼甲素的腹腔注射给药的LD50为168 mg/kg,95%的可信限为149 mg/kg-188 mg/kg。结论:蓝萼甲素的安全性较高,属于低毒药物。

Objective： To investigate the acute toxicity of Glaucocalyxin A in mice and evaluate the safety, to provide the theoretic foundation for deve]opment of new medicine and clinical application. Methods： Aral administration and peritoneal in- jection were applied for the drug delivery. The mortality rates of different dosage of Glaucocalyxin A were observed and the LD50 was determined by Bliss assay. Results： The MTD of Glaucocalyxin A is 2.0 g·kg^-1·d^-1. The LDso of Glaucocalyxin A has not been determined by Aral administration. The dosage of LDs0 of Glaucocalyxin A by peritoneal injection was 168 mg/kg body weight in mice. The 95 % reliable limit is 149 mg/kg-188 mg/kg.Conclusion： Glaucocalyxin A is a low toxic drug with high safety.