苯并(a)芘、多氯联苯和滴滴涕暴露对剑尾鱼肝组织块CYP1A、P-gp mRNA表达的影响

Effect of B(a)P,PCB and DDT on the CYP1 A and P-gp expression in hepatocyte of Xiphophorus helleri

以体外培养剑尾鱼肝组织块为试验材料,研究苯并(a)芘〔B(a)P〕、多氯联苯(PCB)和滴滴涕(DDT)及其混合物对CYP1A、P-gp mRNA表达的影响。试验设6个处理,分别为B(a)P、PCB单独处理组:接受不同剂量B(a)P或PCB(0.5、1、5、10μmol/L)处理6 h;DDT单独处理组:接受不同剂量DDT(0.1、1、10、20μmol/L)处理6 h;B(a)P+PCB、B(a)P+DDT混合暴露组:PCB(1μmol/L)或DDT(1μmol/L)与不同剂量B(a)P(0.5、1、5、10μmol/L)联合染毒6 h;同时设立1‰二甲基亚砜(DMSO)组为溶剂对照组。结果显示,B(a)P单独暴露各组均显著诱导CYP1A mRNA表达,5μmol/L组P-gp mRNA水平与各组差异显著。PCB单独暴露各组CYP1A、P-gp mRNA水平均差异不显著。DDT暴露组10、20μmol/L高剂量组可显著诱导P-gp mRNA表达,各组CYP1A mRNA水平差异不显著。与单独暴露组明显不同的是:B(a)P 10μmol/L+PCB 1μmol/L混合暴露组CYP1A mRNA水平与各组差异显著。混合暴露组P-gp mRNA水平与对照均差异不显著。表明剑尾鱼肝组织块CYP1A、P-gp mRNA水平在单独暴露与混合暴露中呈现不同的表达规律。

The paper studied the effect of Benzo （a）pyrene [B （a）P], Polychlorinated biphenyl and Dichlorodiphenyltrichloroethane, alone or in combination on the cytochrome 1A （CYP1A） and P-glycoprotein （P-gp） expression, with the hepatocyte cultured in vitro of Xiphophorus heUeri. We set the following treatments： the hepatocyte of Xiphophorus heUeri were treated at B （a）P or PCB with the concentrations of 0.5, 1, 5, 10 μmol/L for 6 hours; at DDT with the concentrations of 0.1, 1, 10, 20μmol/L; at B （a）P＋PCB, B （a）P＋DDT with the concentrations of 0.5, 1, 5, 10 μmol/L B （a）P and 1 panol/L PCB or DDT for 6 hours. DMSO was used as solvent control group. The results showed that under the experimental condition, the liver of Xiphophorus helleri were induced with B （a）P, the highest CYP1A and P-S） mRNA expression was detected. There was no significant difference on CYP1A and P-gp mRNA of the groups that PCB was exposed alone. In the DDT exposed group, 10, 20 μmol/L DDT induced P-gp mRNA expression significantly, but there was no significant difference on CYPIA mRNA. The difference between sole exposure groups and mixed exposed groups was significant about CYPlA mRNA. There was no difference on P-gp mRNA between controls and mixed exposure groups. The result condicated that the expression of CYPIA and P-ap mRNA in the sole exposure groups were different from in the mixed exposed croups.