酪蛋白胶体金快速检测卡的研制

Colloidal Gold-Based Immunochromatographic Assay for Rapid Detection of Casein

目的:制备检测乳制品中酪蛋白含量的胶体金快速检测卡。方法:以酪蛋白为抗原和抗酪蛋白多克隆抗体为原料,用硝酸纤维膜和玻璃纤维纸作为载体,制作出检测酪蛋白胶体金快速检测卡。结果:该检测卡检测酪蛋白的灵敏度可达到2.3mg/mL,检测时间10min,重复性和稳定性良好。结论:本实验成功制作出了能检测酪蛋白的快速检测卡。

Objective： To develop a colloidal gold-based immunochromatographic assay for rapid detection of casein. Methods： We used casein and anti-casein polyclonal antibody as raw materials, nitrocellulose membrane and glass fiber paper as a carrier to prepare a rapid test strip. Results： The sensitivity of the test strip was 2.3 mg/mL, and the detection process required 10 min with good repeatability and stability. Conclusion： We have successfully developed a casein test strip.