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CD44＋CD24^-/low样乳腺癌中过表达与其恶性预后的相关性研究

Human breast cancer stem cell maker CD44 ＋ CD24 -/low enriched in basal - like breast carcinoma
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摘要 目的探讨乳腺癌干细胞样标志物CD44＋CD24-/low在基底样乳腺癌（basal—like breast carcinoma，BLBC）中过表达与BLBC恶性预后的相关性。方法在乳腺癌基因表达分型的基础上，根据雌激素受体（ER）、孕激素受体（PR）、人类表皮生长因子受体2（Her-2）免疫表型的表达选取乳腺癌组织四组：管腔A组、管腔B／C组、Her-2高表达组及三阴性组；对三阴性组检测CK5／6、EGFR，分为正常乳腺样型和BLBC型两组；对上述5组进行免疫组化Envision法染色，选用抗体为CD44、CD24，观察CD44＋CD24-/low表型表达并比较BLBC组与其它各组的差异。结果（1）三阴性组共60例，CK5／6和或EGFR阳性者41例（68．3％），确定为BLBC；CK5／6、EGFR阴性者19例（31．7％），确定为正常乳腺样组；（2）CD44＋CD24-/low表型在BLBC组中占78．0％（32／41），相对于管腔A组37．9％（11／29）、管腔B组25．9％（7／27）、Her-2高表达组17．2％（5／29）、正常乳腺样组26．3％（5／21），表达增高并具有显著性（P〈0．05）；（3）所有150例乳腺癌中（可评价145例）具有CD44＋CD24-/low免疫表型者其Ki-67指数增高相对于非CD44＋CD24-/low表型具有统计学差异（P〈0．001）。结论BLBC型乳腺癌表达乳腺癌干细胞样标志物CD44＋CD24-/low显著高于其它各型乳腺癌，CD44＋CD24-/low与BLBC独特的恶性生物学行为相关。 Objective To study the expression of breast cancer stem cell - like markers CD44＋CD24-/lownow in basal - like breast carcinoma（BLBC） and their relation with the prognosis of BLBC. Methods We classified breast carcinomas into 4 groups ：luminal A ,luminal B/C, Her -2 over -expressing and triple -negative subtype with the expressions of ER, PR and Her - 2, respectively. We classified triple - negative subtype into 2 groups ： BLBC and normal breast - like group with the expression of CK5/6 and EGFR, respectively. Immunohisto- chemical（ Envision method）was performed to all of the 5 groups, with the selection of antibodies for CD44 and CD24. Results （ 1 ） triple - negative group were 60 cases in all, expressed CK5/6 and EGFR in 41cases （ 68.3 % ）, which was identified as BLBC ;low expressed CK5/6 and EGFR in 19 cases （ 31.7 % ）, which identi- fied as the normal breast - like group. （ 2 ） 32 cases expressed CD44 ＋ CD24 phenotype in 41 cases of BLBC78.0% （ 32/41 ）, compared with luminal A group of 37.9% （ 11/29 ）, luminal B of 25.9% （ 7/27 ）, Her - 2 over - expression group of 17.2% （ 5/29 ） and normal breast - like group of 26.3% （ 5/21 ） in BLBC （ P 〈 0.05 ）. （ 3 ） In all cases of breast cancer which can be evaluated in 145 cases with CD44＋CD24-/low immun - phenotype, Ki - 67 index was higher and statistically significant （ P 〈 0.05 ）. Conclusion The expression of human breast cancer stem cell markers CD44 ＋ CD24-/Low in BLBC was significantly higher than other types of breast cancer. Then ,we can suppose an association between the prognosis of BLBC and the presence of CIM4 ＋ CD24 -now cell.
作者 于晓宇 耿敬姝 宋鸿涛 冯迪 蒋旸
机构地区 哈尔滨医科大学附属第三医院病理科
出处 《实用肿瘤学杂志》 CAS 2013年第1期60-64,共5页 Practical Oncology Journal
关键词 乳腺癌 干细胞 基底样乳腺癌 CD44 CD24 Breast Carcinoma Stem Cell Basal - like Breast Carcinoma CD44 CD24
分类号 R735.34 [医药卫生—肿瘤]
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实用肿瘤学杂志
2013年 第1期

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