摘要
目的:研究syndecan-1在结肠腺瘤及腺癌中的表达,探讨其与结肠腺瘤及结肠癌的关系。方法:分别采用免疫组织化学染色技术(immunohistochemistry,IHC)以及逆转录-聚合酶链反应技术(reverse transcription polymerase chain reaction,RT-PCR),检测结肠腺瘤、腺癌及正常肠道组织中syndecan-1 mRNA和蛋白的表达,初步分析其与腺癌的临床病理特征的关系。结果:免疫组化及RT-PCR均显示,syndecan-1在正常组织中明显高表达,而腺癌呈明显低表达,腺瘤则介于两者之间,三者之间差异有统计学意义(P<0.05)。结论:syndecan-1主要表达于正常肠黏膜组织,在腺瘤中表达有所减弱,在腺癌中的表达则明显减弱。这提示细胞间粘附的破坏在结直肠的致癌机制中可能起关键作用。
Objective： To investigate the expression of syndecan-1 on colorectal adenoma and adencarcinoma, and to explore its relationship to the progression of adenoma and cancer.Method： Immunohistochemistry and reverse transcription-PCR were used to detect the expression of syndecan-1 mRNA and protein in adenomas, adencarcinomasand normal intestinal tissues. Its expression was evaluated with the relationship to clinicopathological factors.Result： Both immunohistochemistry and RT-PCR showed that syndecan-1 was highly expressed in normal tissues and was lowly expressed in adencarcinomas, and was middly expressed in adenoma.All of these had significant differences （ P〈0.05 ） .Conclusion： Syndecan-1 is expressed in normal tissue, whilethe expression was lower in adenomas and much lower in adencarcinomas.It is indicated that the destroy of adherence may play a key role in carcinogenesis.
出处
《中国医学创新》
CAS
2013年第5期1-3,共3页
Medical Innovation of China
基金
2011年广东省医学科研基金资助项目(编号:A2011532)
