斑马鱼发育过程中DANA相关基因的定量分析

Quantitative Analysis of Expressions of DANA Related Genes during Development of Zebrafish

DANA是广泛分布于斑马鱼基因组中的一个短散在核重复序列家族,通过实时荧光定量PCR相对定量方法,检测与DANA相关基因mul1a、er-Gicp2在斑马鱼整个个体发育过程中的相对表达量。结果显示:mul1a基因在1细胞期到1 000细胞期,即早期卵裂期内表达量差异不明显,从sphere时期到成体时期,其表达量显著性下调,并在shield时期降到最小值,为1细胞时期的0.009倍;er-Gicp2基因从2细胞到成体时期持续下调,到成体时期其表达量只有1细胞时期的0.023倍。

DANA is a family of short interspersed nuclear elements, which widely exists in zebrafish ge- nome. The relative expressions of DANA related genes,mulla and er-Gicp2, were detected in zebrafish ontogenesis by real-time fluorescence quantitative PCR. The results showed that the relative expression of mull a had no obvious difference from 1 cell stage to 1 000 cell stage, but obviously down-regulated from sphere stage and reached the lowest at shield stage, only about 0. 009 times as that of 1 cell stage. The relative expression of er-Gicp2 gene is persistently down-regulated from 1 cell stage to adult,and the rel- ative expression of adult was only 0. 023 times as that of 1 cell stage.