摘要
确定贵州芹菜中总黄酮的最佳提取工艺。研究乙醇溶剂提取芹菜中总黄酮的提取效果,以芦丁含量为指标用分光光度法测定总黄酮含量,并使用正交试验确定最佳提取工艺条件。结果表明,在不同提取条件中,以乙醇浓度40%,提取温度65℃,料液比1∶18(g:mL),提取3次,每次1 h为最佳提取工艺。
The study aimed to investigate the optimum extraction technology for flavonoids compounds from Guizhou celeries. The optimum technology conditions of extracting the total flavonoids were optinfized by using the single-factor test and the orthogonal experiment. The content of total flavonoids was determined with UV- spectrophotometry. The optimum technology conditions of extracting the total flavonoids from celeries were as follows ： the ethanol concentration of 40 %, extraction temperature of 65 %, extraction time of 1 h, and solid- liquid ratio of 1 ： 18 （g： mL）. The study provided a theoretical basis for the development and utilization of celeries. The optimized process was stable, workable and applicable for industrial production.
出处
《食品研究与开发》
CAS
北大核心
2013年第1期31-34,共4页
Food Research and Development
基金
贵州省科技创新人才团队建设项目(黔科合人才团队[2011]4008)
贵阳市科学技术计划项目([2010]筑科计合同字第3-重-1号)
贵阳市低碳科技计划(筑科合同[2011205]5-14号)
关键词
芹菜
总黄酮
提取工艺
正交试验
分光光度法
Apium graveolens L.
total flavonoids
extracting process
orthogonal test
UV-spectrophotometry
