生产纯净水废水培养螺旋藻技术

The Technology of Cultivation Spirulina sp. in Wasterwater of Production Pure Water

以钝顶螺旋藻(Spirulina platensis)869藻株为实验材料,在不同营养条件下,用纯净水生产废水培养螺旋藻,同时和AB培养基作对照比较。实验结果显示:在每升纯净水生产废水中添加0.5g K2HPO4,8 g NaHCO3、VB12浓度为0.15μg/L,作为改良废水(1),在改良废水(1)的基础上,再按照AB培养基配方添加As液和PIV液,作为改良废水废水(2),在光强强度4 000 lx、温度24.8℃,光暗周期为12 h∶12 h的条件下培养7 d,钝顶螺旋藻液的吸光度值分别能够达到1.713和1.886,此时对应的生物量是1.601 g(DW)/L和1.766 g(DW)/L。同样条件下用AB培养基进行培养,藻液的吸光度为1.802,生物量是1.688 g(DW)/L,显示出用废水培养螺旋藻的可行性。

Cultivation Spirulina platensis 869 in the wastewater from production of pure water under different nutrient conditions was studied, for comprehensive utilization of waste water. The experiment results showed that Spirulina platensis 869 cultured in wastewater did not survival, however inoculated in the improved wastewater （ 1 ） and （2）, growed well, when per liter of the wastewater was modified with 0.5 g K2HPO4,8 g NaHCO3, 0.15p.g vitamin B12, as improved wastewater （1）, and referring to the formula of AB medium, added As and PIV solution, to the improved wastewater （1）, as improved wastewater （2）. When the culture conditions of light intensity, temperature, the cycle of light-dark were： 4 000 lx, 24.8 ℃, 12 h ： 12 h, respectively, for 7 days, The absorbance value of the Spirulina platensis medium can be achieved, respectively, 1.713 and 1.866, the corresponding biomass is respectively 1.601g （DW）/L and 1.766 g（DW）/L. While the Spirulina Cultured in AB medium under the same conditions, absorbanee of the medium is 1.802, the biomass is 1.688 g （DW） / L, which indicating the feasibility of cultivation of Spirulina by wastewater.