菊粉分离纯化工艺的研究

A Study on Separation and Purification of Inulin

以菊糖的保留率为指标,考察了除杂实验中菊糖溶液pH、除杂时间、水浴温度的影响,及其脱色实验中活性炭用量、温度、脱色时间等因素对保留率的影响,并利用正交试验对菊糖的除杂脱色工艺进行了优化,最后对产品采取分离纯化试验。试验结果表明,菊芋中菊糖除杂的优化工艺条件为:除杂温度70℃、水浴时间20 min、溶液最适pH=9;脱色的优化工艺条件为:脱色温度90℃、水浴时间20 min、活性炭用量4%(按体积百分含量加活性炭),然后抽滤除去活性炭,对滤液进行真空浓缩后在50℃条件下干燥,在以上综合条件下得到的菊糖纯度可达92%。

This paper studied the purification of inulin in the process of impurity removal and decolorization process. Inulin in the retention rate as an index to study the impurity experiments inulin solution pH value, purify time, water temperature, and the amount of activated carbon decolorization experiment, temperature and time on the decolorization purity, and the use of orthogonal test of the impurity of inulin decolorization process is optimized, the final separation and purification of the test products. The test showed that the Jerusalem artichoke inulin impurity in the optimization conditions were as follows： impurity temperature at 70℃, time for 20 min, the optimum pH was 9 ; detreated optimization conditions were as follows ： detreated temperature at 90 ℃, time for 20 min, the amount of 4 % of activated carbon （according to the size of activated carbon added）, and then leaching of activated carbon to remove the filtrate concentrated under vacuum at 50℃ in the dry conditions in the above integrated inulin purity of up to 92 %.