四种耐药基因蛋白在大肠癌组织中的表达及临床意义

The expression of 4 drug resistant gene proteins P-gp,TS,GST-π and TopoⅡ in colorectal carcinoma and clinical significance

目的:探讨多药耐药基因P-gp、TS、GST-π、TopoⅡ在大肠癌组织中的表达及其临床病理意义。方法:采用免疫组织化学Envision二步法检测345例大肠癌患者组织中P-gp、TS、GST-π、TopoⅡ的表达。结果:P-gp、TS、GST-π、TopoⅡ在大肠癌组织中的阳性表达率分别为75.94%、66.38%、48.12%、53.91%;P-gp与肿瘤的分化程度相关(P<0.05);TS与肿瘤浸润深度相关(P<0.05);TopoⅡ与肿瘤的分化程度、淋巴结转移、临床分期相关(P<0.05);P-gp与TS呈正相关(P<0.05);GST-π与TopoⅡ呈负相关(P<0.05)。结论:P-gp、TS、GST-π、TopoⅡ在大肠癌组织中的表达有一定的相关性,联合检测上述指标可为大肠癌治疗中选择敏感化疗药物及制定个体化治疗方案提供重要依据。

Objective： To explore the expression of P-gp,TS,GST-π and Topo Ⅱ in eoloreetal carcinoma and clinical significance. Methods： Immunohistochemical staining （Envision method） was used to examine the expression in 345 cases of colorectal carcinoma tissues. Results： The positive rate of P-gp,TS,GST-π and Topo Ⅱ expression was 75.94%, 66.38% ,48.12% ,and 53.91% respectively. There was significant correlation between P-gp,expression and degree of differentiation（P〈0.05）; TS expression was related to depth of invasion（P〈0.05）; Topo Ⅱ expression was associated with degree of differentiation ,lymph node metastasis and clinical staging （P〈 0.05）; P-gp expression was positively correlated to TS expression （P〈0.05）; GST-π expression was negatively related to Topo Ⅱ expression （P〈0.05）. Conclusions： The expressions of P-gp, TS, GST-π and Topo Ⅱ in colotectal carcinoma are correlated, combined detection of these markers will be useful for choosing sensitive chemotherapeutic drugs and selecting individual therapeutic regimen in colorectal carcinoma treatment.