三氯乙烯药疹样皮炎患者淋巴细胞亚群与肝功能损害相关分析

Correlation between lymphocyte subsets and liver dysfunction among patients with dermatitis medicamentosa-like of Trichloroethylene

[目的]研究三氯乙烯药疹样皮炎患者外周血淋巴细胞亚群与肝功能损害之间的关系,为探索其发病机制提供线索。[方法]对32例确诊为三氯乙烯药疹样皮炎患者在入院时采血进行外周血淋巴细胞亚群(在流式细胞仪上运用双标法检测)及肝功能检测,比较淋巴细胞亚群与正常参考值的差异,并与肝功能损害观察指标进行相关性分析。[结果]不同皮疹类型三氯乙烯药疹样皮炎患者之间的外周血淋巴细胞亚群比较,差异无统计学意义(P>0.05);患者外周血成熟T淋巴细胞(CD3^+)、CD8^+T淋巴细胞(CD3^+CD8^+)百分数明显升高,CD4^+T淋巴细胞(CD3^+CD4^+)、NK细胞(CD3^-CD_(16+56)^+)、B淋巴细胞(CD19^+)亚群百分数、CD4^+/CD8^+明显降低,差异有统计学意义(P<0.01);谷酰转肽酶与成熟T淋巴细胞(CD3^+)百分数存在正的线性相关关系;胆碱酯酶、白蛋白与淋巴细胞、CD3^+细胞百分数均存在负的线性相关关系。[结论]三氯乙烯药疹样皮炎患者存在细胞免疫和体液免疫功能紊乱;肝功能损害与细胞免疫系统的活跃存在相关关系。

[Objective] The correlation between lymphocyte subsets and liver dysfunction among patients with dermatitis medicamentosa-like of trichloroethylene was evaluated to explore the pathogenesis of this disease. [ Methods ] A total of 32 patients were studied. Their lymphocyte subsets and liver function at hospitalization time were analyzed. [ Results ] The variation of lymphocyte subsets among the patients with different types of skin rashes had no significant difference （P 〉 0.05 ）; compared with reference values, the percentage of patients＋ mature T lymphocyte （ CD3＋ ） and CD8＋T lymphocyte （CD3＋CD8＋） both increased significantly ; the percentage of CD4＋ T lymphocyte （CD3＋ CD4＋）, NK cell（CD3-CD＋166）,and B Lymphocyte subsets （CD19＋） and CD4＋ / CD8＋ all decreased significantly （P 〈 0.01 ）. There was a positive linear correlation between GTP and T lymphocyte （ CD3 ＋ ） and negative linear correlation between lymphocytes or CD3 ＊ ceils and cholinesterase or albumin. [Conclusion ] The patients with dermatitis medicamentosa-like of trichloroethylene had disorder both of cellular and humoral immunity systems; there existed relationship between the liver dysfunction and this cellular immune system disorder.