宫颈癌性别决定基因9基因启动子区甲基化水平检测对宫颈癌的价值研究

Value of Methylation Level Detection of SOX9 Gene Promoter for Cervical Cancer

目的检测宫颈癌性别决定基因9(SOX9)基因启动子区的甲基化水平并探讨其在宫颈癌诊断、治疗及预后评估中的临床意义。方法采用双探针实时定量聚合酶链式反应(PCR)技术检测48例宫颈癌脱落细胞标本(宫颈癌组)和48例正常宫颈脱落细胞标本(正常宫颈组)中SOX9基因启动子区的甲基化水平,比较两组间的差异及其与宫颈癌组各病理分型间的关系。结果宫颈癌组的平均甲基化分值高于正常宫颈组,差异有统计学意义(P=0.000)。宫颈鳞癌SOX9基因启动子区甲基化分值高于腺癌,G3高于G1～G2,有淋巴结转移和脉管癌栓的高于无淋巴结转移和脉管癌栓的,差异均有统计学意义(P<0.05)。结论 SOX9基因启动子区甲基化与宫颈癌的发生、发展密切相关,可用于指导宫颈癌的诊断、治疗和预后评估。

Objective To explore the value of the methylation level detection of SOX9 gene promoter for cervical canc- er diagnosis, treatment and prognostic evaluation. Methods Taqman - based real - time PCR assay was carried out to detect the methylation level of SOX9 gene promoter in 48 cervical cancer scrapings （cervical cancer group） and 48 normal cervical scrap- ings （normal cervix group） , and then the difference between the two groups and the relationship between the metbylation score and cervical cancer pathology were analyzed. Results The methylation score of SOX9 gene promoter in cervical cancer group was significantly higher than that in normal cervix group （P = 0. 000 ） , and significantly higher in cervical squamous cell carcinoma than in adenocarcinoma （P 〈 0. 05） . Meanwhile, the methylation score of SOX9 gene promoter in G3 , with lymph node metas- tisis and vascular cancer embolus than those in G1 and G2 and without lymph node metastisis and vascular cancer embolus （ P 〈 0. 05 ） . Conclusion SOX9 methylation may play a crucial role in the pathogenesis and progression of cervical cancer, and may provide a valuable biomarker in the diagnosis, treatment and prognosis evaluation for cervical cancer.