LED-蓝光对大鼠肝再生TNF-α、IL-6的影响

Effects of blue LED to the amount of TNF-α and IL-6 in liver regeneration in rats

目的探讨LED-蓝光对大鼠肝大部切除肝再生后TNF-α、IL-6含量变化的影响。方法健康雄性Wistar大鼠24只,随机分为3组,假手术组、模型组及LED干预组。采用Higgins改良方法制备大鼠70%肝切除后肝再生的模型,在手术后的不同时间点,检测血清中TNF-α、IL-6的含量变化。结果各实验组动物在手术后1 d血清中TNF-α、IL-6的水平均有升高,模型组和LED干预组的TNF-α、IL-6水平均高于假手术组(P<0.05),且LED干预组的TNF-α、IL-6水平均显著高于模型组;在术后3 d假手术组血清TNF-α、IL-6水平即趋于正常,而模型组和LED干预组TNF-α、IL-6水平达高峰;在术后5 d和7 d,模型组和LED干预组血清中TNF-α、IL-6的水平开始下降,但LED干预组TNF-α、IL-6的水平仍高于模型组。结论LED-蓝光对肝切除后肝再生起明显的促进作用,其机制可能与增强TNF-α、IL-6的含量有关。

Objective To investigate the effects of blue light-emitting diodes（LED） to the amount of TNF-a and IL-6 in the serum of liver regeneration rats after partial hepatectomy. Methods 24 Wistar rats were randomly divided into three groups： Sham group, Model group and LED group. The model was established by partial keratectomy and serum TNF-a and IL-6 levels at different time points were measured with ELISA. Results The levels of TNF-a and IL-6 in every group were increased at 1 d after operation, and the levels of TNF-a and IL-6 in the groups of Model and LED were higher than those of in Sham group, but the levels of TNF-a and IL-6 in LED group were higher than those of in Model group （P〈0.05）. The levels of TNF-a and IL-6 increased gradually and reached a maximum level at 3d post operation in Model and LED group （P〈0.05） and revered to normal in Sham group. Conclusion Blue LED can promote liver regeneration after partial hepatectomy, its effect is possibly related with increasing the amount of TNF-a and IL-6.