培养条件对仙鹤草愈伤组织诱导、增殖及总黄酮含量的影响

Effects of Culture Conditions on Callus Induction,Proliferation and the Total Flavonoid Content of Agrimonia pilosa

以仙鹤草(Agrimonia pilosa Ledeb)无菌苗的子叶为外植体,采用正交设计实验法,研究了不同培养条件对仙鹤草愈伤组织诱导、增殖及总黄酮含量的影响。结果显示:以1/2MS+6-BA 1.0(mg/L)+NAA 0.5(mg/L),在pH 6.5时,愈伤组织诱导率较高,且长势良好;以1/2MS+6-BA 2.0(mg/L)+NAA 1.0(mg/L),在pH 6.0时,愈伤组织增长最快且总黄酮含量最高。

The cotyledons of Agrimonia pilosa Ledeb were used to study the effect of different culture con- ditions on callus induction, proliferation and the total fiavonoid content through the orthogonal design method. The results showed that： under the condition of 1/2MS ＋ 6 -BA 1.0 （mg/L） ＋ NAA 0.5 （mg/L）, at pH 6.5, the callus was induced more easily, and grew well. The condition of I/2MS ＋ 6 -BA 2.0（mg/L） ＋ NAA 1.0（mg/L）, and pH 6.0 was the most proper condition for callus to proliferate. At the same time, the total flavonoid content in callus was the highest under that condition.