崇仁麻鸡PepT1基因多态性与饲料转化率的关联分析

Correlation between the Polymorphism of PepT1 Gene and Feed Conversion Rate in Chongren Spotty Chicken

试验以60～120日龄崇仁麻鸡为试验材料,根据鸡PepT1基因序列设计引物,采用PCR产物直接测序方法检测PepT1基因单核苷酸多态性,并通过对序列碱基的通读以及峰图的鉴定进行基因型分析,探讨PepT1基因多态性与崇仁麻鸡饲料转化率之间的关系。结果共检测到3个SNP位点,且均为沉默突变。其中在PepT1基因外显子4第95处发生了单碱基的改变(G→A),这个突变产生3种基因型(AA,AB和BB),在PepT1基因外显子6第64处和第70处上发现两处突变(分别是由C→G和A→G),且这两处突变具有同一性,这两个突变产生2种基因型(AA,AB)。用POPGENE 32软件分析PepT1基因的多态信息,PV1位点在崇仁麻鸡上表现为中度多态,PV2位点为低度多态位点,且它们均处于Hardy-Weinberg平衡中。通过SPSS 17.0对崇仁麻鸡PepT1基因各SNPs基因型与饲料转化效率进行相关性分析,发现PepT1基因PV1位点的三种基因型和PV2位点的两种基因型与崇仁麻鸡饲料转化效率均没有显著相关性(P>0.05)。

In this study 60 -120 d Chongren spotty chickens were used as the test material and primers were designed according to the sequence of PepT1 gene. Then the single nucleotide polymorphisms of PepT1gene were detected by PCR direct sequencing and its genotype was analyzed by read-through of DNA sequence along with the identification of its peak chart, which aimed to explore the relationship between PepT1 gene pol- ymorphism and feed conversion rate in Chongren spotty chickens. Three SNP loci were found and they were completely silent mutations. A single base was transformed（G→A） on the 95st spot of exon 4 in PepT1, lead- ing to three genotypes： AA, AB and BB. And another two mutations, which were proved identical, were de- tected on the 64st spot and the 70st spot of exon 6 of PepT1 gene （ C→G and A→G, respectively）, leading to two genotypes： AA and AB. By analyzing PepT1 gene polymorphism with POPGENE 32 software, PV1 ＇ s polymorphic locus was moderate and that of PV2 was low, although they were in Hardy - Weinberg equilibri- um. Correlation analysis between SNPs genotype of PepT1 gene and feed conversion rate was carried out through SPSS 17.0 and showed that three genotypes in PV1 and two genotypes in PV2 of PepT1 gene were not significantly correlated with feed conversion rate （P 〉 0.05 ）.