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脐带MSCs对RA患者PBMC中ROR-γt及IL-17表达的影响

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摘要 目的探讨脐带MSCs体外培养对RA患者Th17细胞调节机制的影响。方法收集18例RA患者(RA组)及6例健康志愿者(对照组)清晨空腹静止状态的静脉血,提取外周血单个核细胞(PBMA),分别按1∶1、1∶10、1∶50比例与脐带MSCs混合培养,设单独PBMC培养者为对照。采用RT-PCR、West-bloting法检测PBMC中Th17相关转录因子(ROR-γt)mRNA及蛋白表达,采用流式细胞仪检测IL-17水平。结果与PBMC单独培养者比较,RA组混合培养后RORγt mRNA和蛋白水平均显著下降,尤其是在病情高度活动者,此种抑制效应呈剂量依赖性;RA组病情缓解者和对照组IL-17水平无显著变化,但RA组疾病活动者IL-17水平显著上升,且病情高度活动者的抑制效应呈剂量依赖性。结论脐带MSCs体外培养能影响RA患者的Th17细胞调节机制,此为临床RA的免疫调节治疗提供了依据。
出处 《山东医药》 CAS 2013年第7期43-46,共4页 Shandong Medical Journal
基金 山西省科技攻关社会发展基金项目(20120313025-6)
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