苯并噻二唑诱导番茄对番茄黄化曲叶病毒病的抗性

Resistance to tomato yellow leaf curl virus in tomato induced by benzothiadiazole

以番茄感病品种1479和2300为材料,研究了苯并噻二唑(BTH)诱导番茄对番茄黄化曲叶病毒病的抗性,并测定BTH处理和番茄黄化曲叶病毒(TYLCV)接种对番茄叶片几种防御酶活性的影响。结果表明,0.1～2.0mmol/L BTH能有效诱导番茄产生对番茄黄化曲叶病的抗性,其中0.5 mmol/L BTH处理效果明显,诱导效率可达42.7%。喷施BTH或接种TYLCV均可提高番茄叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)的活性,但诱导并接种处理的植株叶片上述酶活性比只诱导不接种处理高。说明BTH处理可以诱导番茄增强防御酶活性,降低病情指数,增强对番茄黄化曲叶病的抗性。

The resistance to tomato yellow leaf curl virus （TYLCV） induced by benzothiadiazole was detected on resistant tomatoes 1479 and 2300, and the activities of several defense enzymes of tomato after benzothiadiazole spraying or TYLCV inoculation were determined as well. 0. 1-2.0 mmol/L benzothiadiazole showed an induction of TYLCV resistance, and 0.5 mmol/L benzothiadiazole displayed the best effect with induction efficiency of 42.7%. The defense enzymes in tomato seedlings such as superoxide dismutase （SOD） , peroxidase （POD） , polyphenol oxidase （PPO） and phenylalanine amonnialyase （PAL） all increased after benzothiadiazole spraying or TYLCV inoculation, and their activities in tomato treated by both spraying benzothiadiazole and TYLCV inoculation rose faster than those treated by benzothiadiazole spraying only. It indicated that spraying benzothiadiazole on tomato could enhance the activities of defense enzymes, and reduce the disease index significantly, resulting in increase of TYLCV resistance.