无需衍生-超高效液相色谱法快速测定食品中黄曲霉毒素

Rapid determination of aflatoxin in foods by ultra performance liquid chromatography without derivatization

目的:建立无需衍生-超高效液相色谱法快速测定食品中黄曲霉毒素B1、B2、G1和G2的方法。方法:样品用甲醇水提取,提取液经黄曲霉毒素免疫亲和柱净化后,直接经C18色谱柱分离、荧光检测器检测。结果:4种黄曲霉毒素完全分离,AFG2、AFB2在0.010μg/L～2.00μg/L、AFB1在0.04μg/L～8.00μg/L、AFG1在0.10μg/L～20.0μg/L质量浓度范围内具有良好线性关系;最低检出浓度分别为0.04μg/kg(AFB1)、0.10μg/kg(AFG1)、0.01μg/kg(AFB2、AFG2);回收率为78.5%～110.5%,相对标准偏差(RSD)为2.46%～8.81%。结论:该方法简单、快速、灵敏,适用于食品中4种黄曲霉毒素含量的同时测定。

Objective：To establish a rapid method for determination of aflatoxin B1（ AFB1 ）, B2 （AFB2 ） , G, （AFG1） and G2 （AFG2 ） in foods by UPLC without derivatization. Methods： Samples were extracted with methanol -water. The extract was purified by aflatoxin immunoaffinity column and separated with Cls chromatographic col- unto. Aflatoxins were detected with fluorescence detector. Methods ： In the range of 0. 010 μg/L - 2.00μg/L for AFG, and AFB2 ,0.04 μg/L ～8. O0 μg/L for AFB1 and 0.10 μg/L - 20.0μg/L for AFG1, good linear relationship were obtained. The lowest detection limits were 0.04 μg/kg for AFB1 , 0.10 μg/kg for AFG, and 0.01 μg/kg for AFB2 and AFG2, respectively. The recovery rates were in the range of 78.5%～ 110.5%. The relative standard de- viations were 2.46% to 8.81%. Conclusion： This method is simple, rapid and sensitive and could be used for de- tection of four aflatoxin in foods.