快速检测果蔬中肠出血性大肠杆菌EHEC O104方法研究

Study on rapid method for detecting Enterohemorrhagic Escherichia coli O104 in fruits and vegetables

目的:针对德国暴发流行的肠出血性大肠杆菌EHEC O104并结合我国的特点,建立一套科学的快速检测和鉴定果蔬中肠出血性大肠杆菌EHEC O104的快速检测方法。方法:利用ELISA方法作为快速初筛方法,结合传统生化鉴定及PCR检测作为鉴定方法。结果:经特异性和灵敏度检测,方法特异性达到100%,灵敏度达到1.0×101CFU/ml。对市售的50个果蔬样品进行检测,均未检出。结论:利用该方法建立的快速检测果蔬中肠出血性大肠杆菌EHEC:O104标准方法,可以广泛应用在果蔬中的快速检测。

Objective：According to the outbreak of EHEC 0104 strain in Germany, to establish a rapid method for detection of En,terohemorrhagic Escherichia coli 0104 in fruits and vegetables. Methods： ELISA was used for rapid preliminary screening, the conventional biochemical identification method and PCR were employed for identifi- cation. Results： With these above methods, the specificity was 100% , the sensitivity was 1.0 × 101 CFU/ml. Fifty fruit and vegetable samples were all negative for Enterohemorrhagic escherichia coli O104. Conclusion： This method can be widely used for the detection of Enterohemorrhagic Escherichia coli 0104 in fruits and vegetables.