ASO和抗DNA酶B抗体联合检测对风湿病诊断的临床意义

The clinical value of anti-dnase B and antistreptolysin O microtitration test in the diagnosis of rheumatic disease

目的:研究溶血性链球菌感染引起的风湿病患者抗DNA酶B和ASO的血清学变化及意义。方法:收集30例急性风湿热、30例活动期风湿性心脏病、30例风湿性关节炎患者血清,免疫散射比浊法检测其抗DNA酶B和ASO水平。结果:三组患者的ASO阳性率分别为80%、57.3%、53.3%,抗DNA酶B检测阳性率分别为70%、85.7%、60%。除活动期风湿性心脏病组外,其余两组患者的ASO阳性率与抗DNA酶B检测阳性率间的差异无统计学意义(P>0.05)。三组患者的抗DNA酶B和ASO联合检测阳性率分别为90%、92.8、80%,均高于单一检测(P<0.05)。结论:ASO和抗DNA酶B抗体联合检测可提高风湿热诊断阳性率,对风湿热的诊断具有重要的临床意义。

Objective：To observe she changes and the diagnostic values of anti -Dnase B and Antistreptolysin O in serum of rheumatic disease patients caused by group A streptococcal （GAS） infection. Methods： Thirty patients with acute rheumatic fever （ARF） and 30 patients with Rheumatic activity heart disease and 30 patients with Rheu- matic arthritis were included in the present study. Immune scatter nephelometry test was used to detect the level of anti - Dnase B and ASO in serum. Results： The positive rates of ASO in patients with ARF, Rhemnatic activity heart disease and Rheumatic arthritis were 80% , 57.3% and 53.3% , respectively. The positive rates of anti - Dnase B in patients with ARF, Rheumatic activity heart disease and Rheumatic arthritis weT~ 70% , 85.7% and 60%, respectively. Other than the group of Rheumatic activity heart disease, there was no statistical diffe.rcnce be- tween the positive rates of ASO and anti - Dnase B in patients with ARF and Rheumatic arthritis. The positiw~, rates of the joint detection of anti - Dnase B and ASO in patients with ARF, Rheumatic activity heart disease and Rheu- matic arthritis were 90%, 92.8% and 80% , respectively. There was statistically significant difference in the posi- tive rate between joint detection and any single test. Conclusion： The joint detection of anti - Dnase B and ASO could improve the diagnosis of Rheumatic disease and should be of important clinical value in the diagnosis of rheu- matic disease.