蛋白指纹图谱技术在盆腔结核中的诊断价值探讨

Diagnosis of pelvic tuberculosis by proteomic fingerprinting technology

目的探讨血清蛋白指纹图谱技术在盆腔结核诊断中的应用价值。方法应用蛋白指纹图谱技术(PFT)检测盆腔结核患者、健康者各25例,以Ciphergen蛋白芯片3.1.1软件比较,分析血清蛋白峰值,筛选疾病相关标志物并进行统计学处理。结果存在有4个蛋白峰值(8048m/z、5335m/z、11 700m/z和11 683m/z)为高表达;其组成的诊断模型可以很好的鉴别盆腔结核与健康者,总准确率86%(43/50),特异度100%(25/25),灵敏度72%(18/25),差异有显著性(P<0.01)。结论血清蛋白指纹图谱检测技术简便、快速,标本用量少,是筛选盆腔结核特异性标志物的有效手段,将成为盆腔结核早期诊断的指标之一。

Objective Study on the diagnosis of Pelvic tuberculosis using proteomic fingerprinting technology. Meth- od From the 25 clinically diagnosed cases of Pelvic tuberculosis and the same number of the healthy cases. Serum proteins are then captured using WCX2 chips then scanned and analyzed using PBS II chip reader. Result Compa- ring the serum protein data of 25 cases of Pelvic tuberculosis and 25 cases of healthy cases four protein peaks （8048m/z,5335m/z, 11 700m/z, 11 683m/z） are inclined to be from Pelvic tuberculosis, and is significantly dif- ferent from that of healthy cases （ P〈O. O1 ）. This model of diagnosis has an accuracy of 86.0% （43/50） , sensitivi- ty of 72% （18/25）. ,specificity of 100% （25/25）. Conclusion Although the simplicity, speed, and small sample requirements can potentially provide tuberculosis and lung cancer patients with a new non-invasive method of diagno- sis, further research is needed to increase the sensitivity and specificity of detection.