花生抗氧化水解产物制备及其抗氧化活性研究

Preparation and Antioxidant Activities of Peanut Antioxidant Hydrolysate

研究花生抗氧化水解产物,为进一步研究花生抗氧化肽提供理论基础。本研究运用Box-Behnken设计的响应面试验方法对花生抗氧化水解产物的制备工艺条件进行优化,其最佳制备工艺条件为:经复合多糖酶(Viscozyme L)预处理的花生粕粉,调节pH值8.2,加入4000U.g-1底物的碱性蛋白酶(Alcalase),在50.0℃下酶解94.0min。此工艺下制备的花生抗氧化水解产物对1,1-二苯基苦基苯肼(DPPH)清除率为77.38%。花生抗氧化水解产物的抗氧化活性结果显示,对DPPH自由基、羟自由基、超氧阴离子自由基的清除率的IC50值分别为6.65、0.56和7.67mg.mL-1,对铁离子和铜离子螯合率的IC50值分别为8.02和12.39mg.mL-1,对脂质过氧化的抑制率的IC50值为8.45mg.mL-1,铁还原力和钼还原力吸光值为0.5时,所需抗氧化水解产物浓度分别为26.40和4.59mg.mL-1。

The objective of this work is to study on the peanut antioxidant hydrolysate and to provide a theoretical basis for further investigating peanut antioxidant peptide. The technological conditions were optimized by response surface methodology （RSM） based on the Box-Behnken design. Results indicated that the 1, 1-diphenyl-2-pieryl-hydrazyl （DPPH） free radical scavenging activity of peanut hydrolysate could reach 77.38% under the following optimum conditions： initial pH value of 8.2, Alcalase dosage of 4000U·g^-1 substrate, reaction temperature of 50.0 ℃, and incubation time of 94.0min. The antioxidant activities of peanut hydrolysate were as follows. The IC50 values for scavenging of DPPH, hydroxyl, and superoxide anion free radicals were 6.65mg·mL^-1 , 0.56mg·mL^-1 , and 7.67mg·mL^-1 , respectively; For iron and copper ion chelating rate were 8.02mg·mL^-1 and 12.39mg·mL^-1 , respectively; For inhibition rate of anti-lipid peroxidation activity was 8.45mg·mL^-1. The required concentrations for determining iron and molybdenum reduction capacities at absorbance 0.5 were 26. 40mg·mL^-1and 4.59mg·mL^-1, respectively.