七种培养基对黄曲霉分离效果的比较

Comparison of Seven Media for the Isolation of Aspergullus Flavus Group

为了筛选出适宜黄曲霉分离的培养基,比较了7种常用于霉菌分离的培养基对10个花生土壤样品的黄曲霉分离效果,这7种培养基为氯硝胺18%甘油培养基(DG18)、高盐察氏培养基(SCDA)、马铃薯琼脂培养基(PDA)、改良的孟加拉红培养基(M-RB)、YES培养基、孟加拉红培养基(RB)、氯硝胺孟加拉红琼脂(DRBC)。研究发现7种培养基对黄曲霉菌培养效果差别很大,培养效果最好的是DG18,黄曲霉的培养数是PDA的7.3倍。同时DG18能很好的抑制毛霉的生长,与DRBC培养基相比,能抑制81%的毛霉菌生长。因此DG18培养基能最大限度的保证黄曲霉的生长分离,同时也能更好地抑制毛霉的生长,更适用于黄曲霉种群、遗传方面的研究应用。

Seven agar media used to isolate mould were compared for utility in isolating the Aspergillus flavus from peanut-cropped soils. The seven agar media were Dicloran 18% glycerol medium salt capek Dox agar,Patato dextrose agar medium,Modified Rose Bengal medium,YES medium,Rose Bengal medium,Dicloran Rose Bengal medium. The results showed that seven media have apparent difference on isolating Aspergillusflavus. DG18 was the most suitable medium for studying the population biology of this group because it permitted both identification of the greatest number of Aspergillus flavus group strains and growth of the fewest competing fungi. DG18 supported about 7.3times more AspergiUus flavus colonies than the PDA while reducing the number of mucorales colonies by 81% than the DRBC. So DG18 should be the most useful for studies on the population biology of the Aspergillus flavus group.