超微粉碎技术在沙柳原料预处理中的应用

Application of ultrafine jet mill technology in the pretreatment of Salix lignocelluloses

以沙柳为原料,研究了超微粉碎预处理技术对沙柳原料酶水解效果的影响,基于Box-Behnken实验设计,对经过超微粉碎预处理的沙柳原料,采用响应面分析法优化了超微粉碎沙柳原料稀碱预处理的工艺条件,考察了原料粒径,稀碱处理过程中碱浓度、处理时间、处理温度对原料水解的影响,并建立了工艺数学模型。通过实验得到最佳超微粉碎沙柳原料酶解的预处理条件:原料粒度(15μm)、0.79%NaOH、94.6℃、43.4min,原料的酶解率可以达到最大值。结果表明,超微粉碎预处理的沙柳原料经过稀碱预处理后可明显提高纤维素的酶水解效率,该模型为超微粉碎沙柳原料酶解工艺的进一步研究提供了依据。

The pretreatment of lignocellulosic biomass by ultrafine air jet mill technology was investigated using Salixpsammophila as the substrate. Box-Behnken experiment design of the response surface methodology （RSM） was employed to study the effects of particle size, NaOH concentration, reaction time and temperature on the enzymatic hydrolysis of pretreated lignocellulose. A quadratic polynomial equation was obtained for enzymatic hydrolysis by multiple regression analysis using RSM. The results of validation experiments were coincident with the predicted model. The optimum conditions for Salix pretreatment were as followed： particle size 15txm, NaOH 0.79%, 94.6℃ and 43.4min. The Salix hydrolysis rate can reach the maximum yield. The results showed that ultrafine jet mill pretreatment of Salix with dilute NaOH can significantly improve the enzymatic hydrolysis of lignocellulose, The model can provide some reference for the further study of ultrafine pretreatment of Salix hydrolysis.