摘要
利用8个代表性菌株从153对引物组合中筛选出多态性高且稳定性好的10对引物组合,对来自黑龙江省各水稻主要产区的214个稻瘟病菌菌株进行RSAP(Restriction site amplified polymorphism)分析,得到134条多态性条带,经聚丙烯酰胺凝胶电泳,得到196个不同单元型(带型)的代表性菌株的指纹图谱。用UPGMA进行聚类分析,以相似系数0.83为界,将214个菌株196个单元型划分为10个遗传宗谱,10个遗传宗谱在黑龙江省的分布存在明显地域差异。
Total 10 higher polymorphism and good stability pairs of primers were selected from 153 pairs of RSAP primers by using DNA of eight typical isolates of Magnaporthe grisea. Total 134 different polymorphism stripes were obtained after RSAP analysis of 214 Magnaporthe grisea isolates from Heilongjiang Province were done. Total 196 different haplotypes （stripes types） of the representa- tive RSAP fingerprint were aquired by polyacrylamide gel electrophoresis. 0.83 was as the similarity coefficient boundary, 214 strains were divided into 10 genetic lineages by cluster analysis using UPGMA software. Magnaporthe grisea in Heilongjiang Province showed rich genetic diversity, and the distribution of the 10 genetic lineages were obviously area difference.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2013年第1期34-38,共5页
Journal of Northeast Agricultural University
基金
黑龙江省教育厅科学技术研究项目(11551041)
博士后研究人员落户黑龙江科研启动资助项目(LBH-Q09172)
农业部寒地作物生理生态与黑龙江省高校寒地作物品种改良与生理生态重点开放实验室项目
