冷暴露对长爪沙鼠BAT及UCPmRNA的影响

EFFECT OF COLD EXPOSURE ON BAT AND UCPmRNA IN MONGOLIAN GERBIL (MERIONES UNGUICULATUS)

长爪沙鼠随机分为对照组和低温组 .对照组动物生活在 12h光照∶12h黑暗 (12L∶12D) ,(2 5± 2 )℃温暖环境下 ;低温组生活在 12h光照∶12h黑暗 (12L∶12D) ,(4± 2 )℃环境下 .低温组动物又根据暴露时间不同随机分为 7组 :12h组 ,2 4h组 ,3d组 ,7d组 ,14d组 ,2 1d组 ,2 8d组 .与对照组相比 ,长爪沙鼠BAT的体质量和总DNA质量在冷暴露 12～ 2 4h降低 ,而 7～ 2 8d则增加 .长爪沙鼠BAT总蛋白质的质量含量在冷暴露 2 4h时就明显增加 ,并随冷暴露时间的延长继续增加 .低温环境下 ,长爪沙鼠解偶联蛋白 (UCP)的基因表达也比对照组增加 .结果表明 ,冷暴露能够诱导长爪沙鼠的BAT细胞增补 ,UCP基因表达增加 ,从而使其适应性产热增加 .

Mongolian gerbil ( Meriones unguiculatus ) are devided randomly in seven groups in cold temperature(12 L∶12 D),(4±2) ℃ for 12,24 h,3,7,14,21 and 28 d;the control group is in warm temperature(12 L∶12 D),(25±2) ℃. Compared with control group, the weight of BAT and the total contents of DNA in BAT decrease in cold temperature from 12 h to 24 h. During cold exposure from 7 d to 28 d, the indexes above increase significantly. The protein contents of BAT also increase in cold exposure from 1 d to 28 d. In molecular level the contents of UCPmRNA in BAT increase too during cold exposure. In conclusion,cold temperature can induce the recruitment of BAT and the increasement of the expression of UCP gene in Mongolian gerbil. Therefore, the adaptive thermogenesis of Mongolian gerbil is enhanced to adapt to cold temperature.