摘要
为研究养殖循环水系统中分解氨氮能力较强的相关微生物,从生物滤料载体上收集正常运行条件下自然生长的生物膜。通过富集培养、分离提纯、DNA提取、PCR扩增、16SrRNA测序等步骤,得到一株具有分解氨氮能力的荧光假单胞菌Pseudomonas fluorescens SS101,菌落为淡黄色。结果显示,广泛存在能够分解养殖污水中氨氮并用于自身生长的微生物,除了常见的硝化细菌,一些特异性微生物也具有此功能。这项研究为进一步探索养殖污水微生物群落、优化生物滤池的污水处理性能提供了新思路。
In order to study the treatment capacity of marine microorganism in bio-fiher, marine bacteria were separated from the bio-film of activated sludge in the circulating water system. By the enrichment culture, purification, DNA extraction, PCR amplification, 16 SrRNA sequencing, a light yellow strain fluorescent pseudomonad Pseudomonasfluorescens SS101 was got, and the strain number was PRJNA67539. The result revealed that in addition to nitrifying bacteria, some specific microorganism also would decompose ammonia nitrogen for their growth in cultivation wastewater. This study will about the microorganism community in cultivation wastewater, and bio-film in wastewater treatment system. provide basic information on further studies lay a foundation to optimize the property of
出处
《渔业现代化》
北大核心
2013年第1期1-6,共6页
Fishery Modernization
基金
"十二五"国家科技支撑计划项目"节能环保型循环水养殖工程装备与关键技术研究"(2011BAD13B04)
关键词
养殖污水处理
循环水系统
生物膜法
分离培养
16SrRNA
cultivation wastewater treatment
circulating water system
bacterial bio-film
separate culture
16SrRNA
