摘要
目的:观察椎管内注射眼镜蛇毒najanalgesin对大鼠L5脊神经结扎并剪断(spinalnerve ligation and transection,SNL)术后脊髓谷氨酸-天冬氨酸转运体GLAST(glutamate-asparate transporter,GLAST)mRNA表达的影响,探讨najanalgesin脊髓镇痛机制。方法:采用L5SNL模拟神经病理性疼痛,von Frey hair测定大鼠术侧后足50%机械刺激撤足反射阈值(50%paw withdrawal threshold,PWT)判断模型成功,real-time PCR检测SNL 7 d后脊髓L4-L6GLAST mRNA表达。结果:SNL诱导大鼠产生了机械痛敏,表现为50%机械刺激PWT降低,模型复制成功。SNL 7 d后脊髓GLAST mRNA表达降低,椎管内给予najanalgesin剂量相关上调SNL大鼠脊髓GLAST mRNA的表达。结论:Najanalgesin可以增加脊髓GLAST mRNA的表达,可能是其脊髓镇痛机制之一。
Objective： To investigate the effect of intrathecal administration of najanalgesin on mRNA of glutamate-asparate transporters （GI,AST） in spinal cord of rats after 1,5 spinal nerve ligation and transaction （SNL）, and Io explore the spinal analgesic mechanism of najanalgesin. Methods： An L5 SNL model was sel up, and lhe 50% paw withdrawal threshold （PWT） was detected by yon Frey hair to evaluate the replication of animal model. The mRNAs of GLAST in the IA-L6 segments of the spinal cord of SNL rats after 7days were determined by real-time PCR. Results： Mechanical hyperalgesia was induced after SNL with decreased 50% PWT, indicating that the L5 SNL animal model was successfully replicated. The level of GLAST mRNA was decreased after 7 d of SNL. lntrathecal administration of najanalgesin could dose- dependently upregulate the expression of GLAST mRNA in spinal after SNL. Conclusion： Najanalgesin can increase the expression of GLAST mRNA in spinal cord, which may be one of its mechanisms in spinal analgesia.
出处
《药学与临床研究》
2013年第1期21-25,共5页
Pharmaceutical and Clinical Research
