酶光度分析法测定痕量亚硫酸盐

Enzyme spectrophotometric determination of trace sulfite

在pH =3.6的NaAc HAc介质和亚硫酸盐氧化酶存在下 ,亚硫酸盐被氧化成SO4 2 - 及H2 O2 ,在过氧化氢氧化酶作用下 ,用 3,3′ ,5,5′ 四甲基联苯胺 (TMB)显色 ,在 6 50nm波长下间接测定亚硫酸盐的含量 ,线性范围为 0 .15～ 2 0 μg/mL ,可用于食品中亚硫酸盐的测定 .

In the solution of NaAc HAc (pH=3.6) and sulfite oxidase, sulfite can be oxidized to form SO 4 2- and H 2O 2 , and then colored by horseradish peroxidase and 3,3′,5′5′ tetramethylbezidine (TMB). The enzymatic method was used for measuring sulfite in the food with the linear range 0.15～20μg/mL at 650nm.