山西安泽青翘中总蛋白质氨基酸和微量元素分析

Total protein,amino acids and trace elements in green fructus forsythiae from Anze,Shanxi province

目的测定山西安泽青翘的总蛋白质、氨基酸、微量元素含量,对其营养价值进行评价,为综合开发利用青翘资源提供依据。方法采用凯氏定氮法测定总蛋白质,氨基酸自动分析仪测定氨基酸组分,原子吸收法测定钙(Ca),铁(Fe),锌(Zn),铜(Cu),铅(Pb)。结果测得青翘总蛋白含量为6.87%,青翘含有17种氨基酸,氨基酸总量为6.54%,其中人体必需氨基酸总量为2.57%,占总氨基酸的39.20%。青翘含有Ca,Fe,Zn,Cu微量元素,其中Ca为2380μg/g,Fe为188μg/g,Pb未检出。结论山西安泽青翘含有较丰富的蛋白质、多种氨基酸和微量元素,为具有营养价值的保健及药用原料,具有良好的开发利用前景。

Objective To determine the total protein, amino acid and trace elements in green Fructus forsythi- ae from Anze, Shanxi province, and to assess the nutritional composition for offering the rationales for development and utilization in a comprehensive manner. Methods The total protein was assayed by Kjeldahl＇s approach, the amino acid components were assessed by an automatic analyzer and the trace elements （Ca, Fe, Zn, Cu and Pb） were detected by atomic absorption spectrometry, respectively. Results The content of total protein was 6.87% and the to- tal content of amino acids 6.54%. Seventeen amino acid components were identified in green Fructus forsythiae, in which the content of essential amino acids accounted for 39.20% of all amino acids and 2.57% as required for the body. The trace elements comprised of calcium （2380 Ixg/g）, iron （188 pLg/g）, zinc and copper. Lead was not detected. Conclusion Supplemented with an abundance of protein, amino acids and trace elements, green Fructus forsythiae from Anze, Shanxi province may be the raw material for health care and medical purposes and has considerable poten- tial to be further explored.