谷氨酰胺对肝硬化大鼠肠屏障功能的保护作用

Protective effects of glutamine on intestinal barrier in rats with liver cirrhosis

目的观察谷氨酰胺对肝硬化大鼠肠屏障功能是否具有保护作用。方法采用CCL4+酒精制备大鼠肝硬化模型。30只Wistar大鼠随机分为正常对照组,肝硬化模型对照组及谷氨酰胺治疗组。使用苏木素-伊红(HE)、胶原纤维(VG)染色以及电镜技术从形态学上检测谷氨酰胺对肝硬化程度以及肠功能屏障障碍的改善状况,采用血浆二胺氧化酶和血浆内毒素水平检测各干预组的疗效。结果 VG染色显示谷氨酰胺治疗组肝纤维化程度减轻;HE、电镜结果显示谷氨酰胺治疗组肠屏障结构损伤减轻;门静脉内毒素水平及血浆二胺氧化酶(DAO)水平在肝硬化模型组均显著高于正常对照组(P<0.05),谷氨酰胺治疗组两指标水平均低于同期肝硬化模型组(P<0.05)。结论谷氨酰胺对肝硬化大鼠肠屏障功能具有保护作用。

Objective To determine the protective effects of glutamine on intestinal barrier in rats with liver cirrhosis. Methods Following establishment of the liver cirrhosis model induced by carbon tetrachloride and alco- hol, 30 Wistar rats were randomly assigned to normal control, liver cirrhosis model control and glutamine treatment group, respectively. The amelioration in liver cirrhosis and intestinal barrier dysfunction following administration of glutamine was assessed by HE and VG staining and electron microscopy. The levels of plasma diamine oxidase and endotoxin were employed to evaluate the efficacy in respective group. Results It has been evidenced by VG staining that glutamine treatment group was characterized of mostly attenuated liver fibrosis and by HE staining and electron microscopy that intestinal barrier dysfunction was reduced. Higher level of portal vein endotoxin and plasma DAO was noted in cirrhosis model group normal, but not in control group （P〈0.05）. Conclusion Glutamine plays a protective role on the intestinal barrier in rats with liver cirrhosis. Furthermore, the two indicators in treatment group were lower than those in cirrhosis model group at the same period （P〈0.05）.