胃肠间质瘤危险性分级与哺乳动物雷帕霉素靶蛋白和磷酸化哺乳动物雷帕霉素靶蛋白表达的关系

Relationship between the risk grade of gastrointestinal stromal tumor and expression of mammalian target of rapamycin and phosphorylated mammalian target of rapamycin

目的研究胃肠间质瘤危险性分级与磷脂酰肌醇3激酶(PI3K)/蛋白激酶(Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)信号传导通路中mTOR和磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)表达的关系。方法用免疫组织化学法检测40例胃肠间质瘤中PI3K/Akt/mTOR信号传导通路相关蛋白mTOR和p-mTOR的阳性表达率,分析其在不同危险程度组之间的差异。结果 mTOR、p-mTOR蛋白在部分胃肠间质瘤呈阳性表达,定位于细胞质。mTOR、p-mTOR在极低和低度危险组的阳性表达率均低于中高度危险组,差异均有统计学意义(P<0.05)。结论胃肠间质瘤PI3K/Akt/mTOR信号传导通路中mTOR和p-mTOR与其侵袭危险性有关,提示该信号传导通路在介导胃肠间质瘤发生、发展过程中起重要作用。

Objective To explore the relationship between the risk grade of gastrointestinal stromal tumor（GIST） and the expression of mammalian target of rapamycin （ mTOR ） and phosphorylated mammalian target of rapamycin （ p-mTOR ） in signal transduction pathway of phosphatidylinositol 3-hydroxy kinase/protein kinase B/mTOR （PI3K/Akt/mTOR）. Methods The positive expression rates of PI3K/Akt/mTOR signal transduction pathway associated protein of mTOR, p-mTOR in 40 patients with GIST was detected by the immunohistochemistry staining. Results The mTOR and p-mTOR were positive ex- pression in some GIST patients and them were localized in the cytoplasm. The positive expression rates of mTOR and p-mTOR in very low-risk and low-risk group were significantly lower than those in intermediate-risk and high-risk group （ P 〈 0.05 ）. Conclusion There expression of PI3K/Akt/mTOR signal transduction pathway associated protein of mTOR, p-mTOR is relat- ed to risk grade. It suggested that this signal transduction pathway may play an important role in the development process in GIST.