期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

E2F-1基因对HepG2肝癌细胞增殖影响的研究 被引量：2

Effects of E2F-1 on Proliferation of Hepatocellular Carcinoma Cell Line HepG2
下载PDF
导出
摘要 目的了解转录因子E2F-1对肝癌细胞HepG2增殖的影响。方法脂质体Lipofectami-neTM2000将pCMV-E2F-1-HA2载体转染HepG2肝癌细胞,然后用含G418的培养液筛选获得具有Genectin抗性的过表达E2F-1的肝癌细胞株。通过MTT法和克隆形成实验检测细胞增殖的变化。结果 MTT和克隆形成实验结果显示,过表达E2F-1的HepG2/E2F-1细胞组与HepG2/EV细胞组和HepG2细胞组相比,其增殖速度明显减慢(P<0.01)。结论 E2F-1基因过表达可抑制HepG2肝癌细胞的增殖。 Objectlve To investigate the effects of over-expression of E2F-I on proliferation of hepato- cellular carcinoma. Methods The eukaryotic expression vector pCMV-E2F-1 -HA2 was stably transfected in- to HepG2 cell by LipofectamineTM 2000. After that, genectin-resistant E2F-1 overexpressed ceils were gath- ered by culture liquid containing O418. The effect of E2F-1 overexpression on the proliferation of HepG2 cells was observed by colony formation assay and MTT method. Results The proliferation of E2F-1 overex- pressed HepG2/E2F-1 cells was significantly lower than those of HepG2 cells and HepG2/EV cells （ P 〈 0.01 ）proved by MTT and clone formation test. Conclusion Over-expression of E2F-1 gene suppresses HepG2 proliferation.
作者 王长青 吴江 周娟
机构地区 深圳市宝安人民医院肿瘤内科
出处 《医学综述》 2013年第4期722-724,共3页 Medical Recapitulate
基金 广东省深圳市科技计划项目(200903185)
关键词 E2F-1 肝肿瘤 细胞周期 细胞增殖 E2F-1 Liver tumors Cell cycle Proliferation
分类号 R735.7 [医药卫生—肿瘤]
  • 相关文献

参考文献10

  • 1Jemal A,Siegel R,Ward E,et al. Cancer statistics,2008 [ J]. CA Cancer J Clin,2008,58(2) :71-96.
  • 2Kitisin K, Pishvaian MJ, Johnson LB, et al. Liver stem cells and molecular signaling pathways in hepatocellular carcinoma[ J ]. Gas- trointest Cancer Res ,2007,1 (4 Suppl 2) : S13-S21.
  • 3Bell LA, Ryan KM. Life and death decisions by E2F-1 [ J ]. Cell Death Differ,2004,11 (2) :137-142.
  • 4Nakajirrm T,Yasui K,Zen K,et al. Aetivation of B-Myb by E2F1 in hepatocellular carcinoma[J]. Hepatol Res,2008,38(9) :886-895.
  • 5Dong YB, Yang HL, Elliott MJ, et al. Adenovirus-mediated E2F-1 gene transfer sensitizes melanoma ceils to apoptosis induced by to- poisomerase 1I inhibitors [ J]. Cancer Res ,2002,62(6) : 1776-1783.
  • 6Itoshima T, Fujiwara T, Waku T, et al. Induction of apoptosis in hu- man esophageal cancer ceils by sequential transfer of the wild-type p53 and E2F-1 genes:involvement of p53 accumulation via ARF- mediated MDM2 down-regulation [ J ]. Clin Cancer Res, 2000,6 (7) :2851-2859.
  • 7Agniswamy J, Fang B, Weber IT. Conformational similarity in the activation of caspase-3 and-7 revealed by the unliganded and in- hibited structures of caspase-7 [ J ]. Apoptosis, 2009, 14 ( 10 ) : 1135-1144.
  • 8Nguyen KH, Hachem P, Khor LY, et al. Adenoviral-E2F-1 radio- sensitizes p53wild-.type and p53 null human prostate cancer cells [ J]. Int J Radiat Oncol Biol Phys ,2005 ,63 (1) :238-246.
  • 9Wu Z,Yu Q. E2F1 -mediated apoptosis as a target of cancer therapy [J]. Curt Mol Pharmacol,2009,2(2) :149-160.
  • 10Gomez-Gutierrez JG, Garcia-Garcia A, Hao H, et al. Adenovirus- mediated expression of truncated E2F-1 suppresses tumor growth in vitro and in vivor.ll. Cancer.2010.116(18) 4420-4432.

同被引文献16

  • 1Kovesdi I, Reiche! R, Nevins J R. Identification of a cellulartranscription factor involved in E1A trans-activation [ J ]. Cell, 1986, 45(2) :219 -28.
  • 2Choi M Y, Chan C C, Chan D, et al. Biochemical consequences of sedlin mutations that cause spondyloepiphyseal dysplasia tarda [J]. BiochemJ, 2009, 423(2) :233 -42.
  • 3Johnson D G, Schwarz J K, Cress W D, et al. Expression of tran- scription factor E2 F1 induces quiescent cells to enter S phase [J]. Nature, 1993, 365 (6444) : 349 - 52.
  • 4Ferreira R, Naguibneva 1, Mathieu M, et al. Cell cycle-depend- ent recruitment of HDAC-1 correlates with deacetylation of histone H4 on an Rb-E2F target promoter[J]. EMBO Rep, 2001, 2 (9) :794 -9.
  • 5Mundle S D, Saberwal G. Evolving intricacies and implications of E2FI regulation[J]. FASEB J, 2003, 17 (6) :569 -74.
  • 6Sharma N, Timmers C, Trikha P, et al. Control of the p53- p21C1PI Axis by E2fl, E2f2, and E2f3 is essential for G1/S pro- gression and cellular transfornmtion[ J]. J Biol Chem, 2006, 281 (47) :36124 -31.
  • 7Picketing M T, Stadler B M,Kowalik T F. miR-17 and miR-20a temper an E2Fl-induced GI checkpoint to regulate cell cycle pro- gression[J]. Oneogene, 2009, 28(1):140-5.
  • 8康娟,刘瑶,吴小翎,王志刚,许川山,李攀,汪朝霞,陈松.载多西紫杉醇脂质微泡超声造影剂的制备及其性质[J].中华超声影像学杂志,2008,17(8):724-727. 被引量：7
  • 9康娟,吴小翎,张勇,冉海涛,王志刚.载多西紫杉醇脂质微泡联合超声靶向微泡破裂对兔VX2肝癌微血管的作用[J].中国介入影像与治疗学,2011,8(5):431-434. 被引量：11
  • 10刘向真,王如美.乳腺癌组织中E2F-1和Skp2的表达及意义[J].现代肿瘤医学,2012,20(11):2286-2288. 被引量：5

引证文献2

二级引证文献2

医学综述
2013年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部