反相高效液相色谱法测维生素A含量

RP-HPLC Determination of Vit.A

目的：建立一种用反相高效波相色谱测定维生素Ａ的方法。方法：色谱柱：Ｈｙｐｅｒｓｉｌ ＯＤＳ（１５０ｍｉｎ×４６ｍｍ），流动相：甲醇－水（９６：４），流速１．０ｍｌ／ｍｉｎ，紫外检测器波长３２５ｎｍ，柱温２５℃，外标法测定。结果：维生素 Ａ在 ２．５～３０．０μｇ／ｍｌ之间有良好的线性关系，最低检测浓度为 １．５μｇ／ｍｌ，平均回收率为９８．５％，精密度ＲＳＤ±ｌ、５６％（ｎ＝３）。结论：本方法准确、灵敏、适应面广。

Objective: To develop a RP-HPLC for the determina- tion of Vit.A.Methods:The extract was enalyzed on Hypersil ODS (l50mm×4.6mm)column with a mobil phase consisting of 96 vols of methanol and 4 Vols of water at 25℃. The flow rate was 1.0mL/min.The wavelength for measurement was 325nm and quantitated by extrnal standard method. Results: The linearity of method was in the range of 2.5~30.3μg/mL for Vit. A. The limit of determination was l.5μg/mL. Average recoveries vere 98^5%, perxision of determination were l.56%. Conclusion: This method is accurate, sensitive and wide enough to be utilized for the investigation.