HPLC法测定荧光增白剂CBS-127的含量

Determination of the Component of Fluorescent Whitening Agent CBS-127 by HPLC

建立了高效液相色谱法测定荧光增白剂CBS-127含量的方法.采用Sunfire C18(4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-水(90%∶10%),流速为1.0 mL/min,紫外检测波长为356 nm,柱温40℃.CBS-127在0.5～2.5μg范围内有良好线性关系(r=0.999 9);加样回收率为99.78%,RSD值为0.7%(n=6).该方法精密度高、重现性好、结果准确可靠,为荧光增白剂CBS-127的质量评价提供了有效的方法.

The method for determinating the content of Fluorescent Whitening Agent CBS-127 by HPLC was established. Sunfire C18 column（4.6 mmxl50 mm, 5μm） was used with methanol-water（90% ：10%） as the mobile phase. The flow rate of the mobile phase was 1.0 mL/min. The detection wavelength was 356 nm. The column temperature was 40 ℃. There was good linear relationship in the range of 0.5-2.5 μg （r=0.999 9）. The recovery rate was 99.78% and RSD value is 0.7% （n=6）. This method is high accuracy, good reproducibility, reliable results, which is an effective method for the Fluorescent Whitening Agent CBS-127.