电化学发光猝灭法检测环孢霉素A

Determination of Cyclosporine A by Electrochemiluminescent Method

[目的]建立用静态电化学发光分析法检测环孢霉素的方法。[方法]以实验室自合成的羧基钌为发光试剂,环境友好型二丁基乙醇胺为共反应试剂,用静态电化学发光分析法检测环孢霉素。[结果]在pH为6.0的0.06 mol/L PBS缓冲溶液中,羧基钌发光强度的淬灭值与环孢霉素A浓度在9.3×10-9～4.0×10-7mol/L之间呈良好的线性关系(R2=0.995 0),检测限达5.4×10-7mol/L。[结论]该方法是首次用电化学发光方法应用于环孢霉素的检测,为电化学发光方法检测环孢霉素提供了理论与试验依据。

[ Objective ] A static electroehemiluminescent approach was developed to determine cyclosporine A. [ Method] By using the syn- thetic carboxyl ruthenium as luminescent reagents and environmental friendly dibutyl ethanolamine as the co-reaction reagent, cyclosporine was determined by static electrochemiluminescence analysis. [ Resuh ] Linear responses in chemiluminescence quenching of carboxyl ruthenium and concentration of cyclosporine A were good linearity （R2 = 0.995 0） between 9.3x10^-9 mol/L and 4.0 x 10 ^-7 mol/L in 0.06 mol/L PBS buffer solution （ pH 6.0）. The corresponding limit of detection （LOD） was 5.4 x 10 ^-7 mol/L. [ Conclusion] The method is suitable for the determination of cyclosporine by electrochemiluminescence for the first time and provides theoretical and experimental supports