丹参注射液山莨菪碱联合应用预防脂肪栓塞综合征的实验研究

Experimental study on Radix Salviae Miltiorrhizae Injection combined with anisodamine for preventing fat embolism syndrome

目的探讨丹参注射液山莨菪碱联用对脂肪栓塞综合征(FES)的预防作用。方法选择雄性健康SD大鼠100只,按随机数字表法将大鼠分为4组,每组25只。丹参注射液组给予丹参注射液0.4mL/kg;山莨菪碱组给予山莨菪碱2mg/kg;联用组给予丹参注射液0.2mL/kg加山莨菪碱1mg/kg加油酸(OA)0.2mL/kg;模型组给予OA 0.2mL/kg,比较4组用药前及用药后1、2、3、4h大鼠行动脉血氧分压(PaO2)、血浆肿瘤坏死因子-α(TNF-α)及血清游离脂肪酸(FFA)等结果。结果模型组、丹参注射液组、山莨菪碱组与联用组的PaO2、TNF-α、FFA同时点比较差异有统计学意义(P<0.05)。结论丹参注射液与山莨菪碱联合应用预防脂肪栓塞综合征的效果优于两种药物单独应用。

Objective To investigate the preventive effects of Radix Salviae Miltiorrhizae（RSM） Injection combined with aniso- damine on fat embolism syndrome（FES）. Methods 100 healthy male SD rats were selected and randomly divided into four groups according to the randomized digital table,25 cases in each group. The RSM Injection group（A） was given RSM Injection 0.4 mI./ kg,the anisodamine group（B） was given anisodamine 2 mg/kg, the combination group was given RSM Injection 0. 2 mL/kg com- bined with anisodamine 1 mg/kg and oleic acid（OA） 0.2 mL/kg,and the model group was given OA 0.2 mL/kg. The FES model was induced by tail vein injection of OA 0.2 mL/kg. PaO2 ,tumor necrosis factor-α（TNF-α） and serum free fatty acid（FFA） were measured before medication and at 1,2,3,4 h after medication. The morphological and pathological changes of the lung were ob- served. The results were compared among 4 groups. Results The differences of PaO2 ,TNF-α and FFA at the same time point in the group D,A,B and C were statistically significant（P〈0.05）. Conclusion The effects of RSM Injection combined with anisodamine for Dreventing FES is superior to single use one of two drugs.