缬沙坦的血药浓度测定

Determination of Plasma Concentration of Valsartan by HPLC

测定了人血浆中的缬沙坦的药物浓度。以替米沙坦为内标,血浆样品采用液-液萃取法提取,高效液相色谱法测定。色谱柱:Agilent ZORBAZX Eclipse XDB-C18柱(4.6×150 mm,5μm);流动相:乙腈:0.01mol.L-1KH2PO4缓冲液(pH=2.96,48:52);流速:1.0 mL.min-1;检测波长:激发波长265 nm,发射波长378 nm;柱温:30℃。结果缬沙坦在12.5～5 000 ng.mL-1浓度范围内线性良好(r=0.997 3),平均提取回收率为58.7%,日内、日间精密度RSD为5.13%～7.93%。血浆样品在-20℃反复3次冻融、预处理后室温放置24 h、-20℃冰冻放置1个月后3种不同考察下稳定性均良好。结论本方法准确、快速,适用于缬沙坦的血药浓度测定及药代动力学研究。

Telmisartanas was used as intemal standard, plasma samples were extracted with a liquid-liquid method, then valsartan concentration in human plasma was determined by HPLC. Stationary phase： Agilent ZORBAZX Eclipse XDB-C18 column （4.6×150mm,5μm）; Mobile phase： acetonitrile and 0.01 mol.Ll KH2PO4 buffer （ pH 2.96, 48 ： 52）; Flow rate： 1.0 mL·min^-1; Detection wavelength： excitation wavelength 265 nm, Emission wavelength 378 nm; Column temperature： 30 ℃. The results show that the method has better linear when valsartan concentration is in the range of 12.5 to 5 000 ng·mL^-1（ r = 0.997 3）, the average extracted recovery is 58.7%, and the precision RSD is in the range of 5.13% - 7.93%. The stabilities of plasma samples are excellent. This method is simple, accurate and reliable. It is suitable for detection of the concentration ofvalsartan in human blood samples so as to study its pharmacokineties.