安徽部分地区鸡柔嫩艾美耳球虫单卵囊的分离与鉴定

Single-oocyst Separation and Identification of Eimeria tenella in Chickens in Patrs of Anhui Province

为获得纯种柔嫩艾美耳球虫虫株,从安徽省巢湖市、霍邱县、定远县3个地区患球虫病的鸡场采集3株混合球虫,培养至孢子化,分离单卵囊接种1～7日龄的雏鸡,感染后7～12 d,收集卵囊,然后通过生物学特性和PCR方法进行虫种鉴定。结果显示,3个地区的单卵囊感染成功率分别为50%、57%和29%,各虫株经生物学特性和PCR鉴定均为柔嫩艾美耳球虫。研究表明:1.0%琼脂单卵囊分离法简便且成功率较高,利用特异性引物PCR鉴定虫株,为纯种地方株的研究奠定了基础。

In order to obtain pure Eimeria strain, three Eimeria species were collected from the poultry farms infected with coccidia in Chaohu, Huoqiu and Dingyuan of Anhui Province, and then cultured to sporu- lated oocysts. The 1-7 days old chicks were vaccinated the single-ooeyst by single-oocyst separation tech- nology. The oocysts were collected after 7-12 days, and then the coccidia ooeysts were identified with biolog- ical characteristics and PCR method. The results showed that the ratio of infection were 50%, 57% and 29%, respectively in three areas. The three Eimeria species were all identified as Eimeria tenella by biological characteristics and PCR. This indicated that the single oocyst isolation technique was simply and successful. Using specific primers of PCR to identify strains could lay foundation for the study of pure local strains.