木聚糖酶高产菌株的筛选及固态发酵条件的研究

Isolation of a high yield xylanase fungus and studies on its solid state fermentation

从 6株黑曲霉菌株中筛选到一株产木聚糖酶活力最高的黑曲霉M1菌株 (AspergillusnigerM1) ,其最适固态发酵条件为 :选择 70 %玉米芯粉 + 30 %麸皮 + 0 6 % (NH4 ) 2 SO4 作培养基 ,其加水质量比为 1∶2 0 ,30℃培养 84h ,其木聚糖酶活力最高可达 3 6 89μmol·min-1·g-1.

A high yield xylanase fungus Aspergillus niger M1 was isolated and its solid state fermentation conditions were studied.The optimal culture conditions were as follows:corncob meal:wheat bran=7∶3,0.6% of ammonium sulfate,dry medium:water=1∶2.0,temperature 30℃ and cultivation for 84 hours.The xylanase activity was as high as 3 689 μmol·min -1 ·g -1 in dry culture.