基质分散萃取—反相高效液相色谱法测定猕猴桃中氯吡脲

Determination of Forchlorfenuron in Kiwi Fruit by Matrix Solid Phase Dispersion Extraction and Reversed High Performance Liquid Chromatography

建立了基质分散萃取-反相高效液相色谱法测定猕猴桃中氯吡脲的方法:以猕猴桃为研究材料,乙腈为提取剂,甲醇为淋洗液、经氨基柱净化后,经高效液相色谱检测。结果表明:以甲醇/水(67/33,V/V)1mL.min-1为流动相,经Kromasil C18(250mm×4.6mm,5μm锈钢柱、UV265nm进行待测组分的分离和测定效果最佳,检测线性范围0.025～100μg.mL-1,相关系数r=0.999 9,添加低、中、高浓度的氯吡脲回收率分别为108%、98.3%、97.9%,相对标准偏差分别为2.1%、0.27%、0.23%,方法检出限为0.01mg.kg-1。该方法测定猕猴桃氯吡脲含量准确可靠。

Forchlorfenuron,a residue of plant growth regulator in kiwi fruit was determined by matrix solid phase dispersion extraction（MSPD） and reversed high performance liquid chromatography（RP-HPLC）.Kiwi fruit was extracted with acetonitrile.The extract was pre-purified by amino column eluted by methanol.The resultant extract was then separated and measured by HP LC.The optimum conditions of HPLC were as follows： mobile phase： methanol/water（67/33,V/V） with a flow rate of 1.0 mL·min-1,column： Kromasil C18（250 mm×4.6 mm,5 μm）,and UV detection wavelength：265 nm.External standard calibration was adopted for quantification.The linearity was good when the concentration ranged from 0.125 to 100 μg·mL-1（r=0.999 9）.Recovery rates at low,medium and high concentrations of the addition of forchlorfenuron were 108%,98.3%,and 97.9%,with the relative standard deviations of 2.1%,0.27%,and 0.23%,respectively.The limit of detection was 0.01 mg·kg-1.The method is accurate and reliable for the determination of forchlorfenuron in kiwi fruit.