建立高质量马铃薯细胞悬浮培养物的研究

STUDY ON THE ESTABLISHMENT OF HIGH QUALITY CELL SUSPENSION CULTURE IN POTATO

以马铃薯四倍体栽培品种甘农薯２号、布尔班克（Ｒｕｓｓｅｔ Ｂｕｒｂａｎｋ）的块茎和茎段两种外植体为材料，进行了愈伤组织诱导和继代培养、细胞悬浮培养物的建立及其继代培养的研究。结果表明：选取良好的愈伤组织经１～３次继代培养后进行细胞悬浮培养，培养物每５ｄ继代一次，继代时新旧培养基体积按３：１配置，能获得较高质量的培养物。在悬浮培养及继代培养过程中，用高浓度蔗糖溶液或蒸馏水调节该培养液的渗透压，以及采用与愈伤组织固体培养基成分相同的细胞悬浮培养基和悬浮继代培养基，可有效地控制悬浮细胞的质壁分离和破碎现象，有助于建立高质量的细胞悬浮培养物。

Two explants (tuber discs and stem segments) from 2 potato tetraploid culitivars (Gan- nongshu No. 2 and Russet Burbank) of Solanum tuberosum were used as the dornor. The experiments of cal- lus induction and subculture, establishment of cell suspension culture and cell suspension subculture were carried out. The results showed that the good quality suspension could be obtained by selecting the calli from the sub- cultures for 1-3 times, subculturing the suspension for every 5d and refreshing the culture media with 3/4 the same kind newly prepared media. When high concentration sucrose or sterile distilled water was used to adjust the medium osmotic pressure in cell suspension culture, and the same medium was used in callus culture, cell suspension culture and cell suspension subculture, the problems of cell plasmolysis and breakage could be solved effectively. These results are beneficial to establishing the cell suspension culture system with high quality.