摘要
目的对3种临床常用的丙型肝炎检测方法的结果进行比较和分析,探讨其临床应用价值。方法对2010年8月—2011年4月临床收集的328例标本(其中105例临床确诊为丙型肝炎),分别应用逆转录聚合酶链反应(RT-PCR)技术定量检测丙型肝炎病毒(HCV)-RNA,酶联免疫吸附试验(ELISA)和胶体金免疫层析法检测HCV-Ab。结果在105例确诊标本中RT-PCR法检测出阳性结果为77例,ELISA法为81例,胶体金法为82例,3种方法联合检测为92例;在223例非丙型肝炎病人中假阳性结果,RT-PCR法为1例,ELISA法为43例,胶体金法为48例。结论 HCV-RNA定量检测的特异性较高,而ELISA法及胶体金法检测HCV-Ab假阳性率较高,3种检测方法的联合应用能够提高HCV的阳性检出率,并减少假阳性。
Objective To compare and analyze the results of three commonly used test methods for hepatitis C and assess their value in clinical application. Methods From 2010 08 to 2011-04, 328 clinical samples were collected (105 cases were con firmed as having hepatitis C), the serum HCV RNA was detected by real-time PCR (RT-PCR), and HCV-Ab by enzyme linked immunosorbent assay (ELISA) and immunocolloidal gold test. Results Among 105 patients, the positive rates were 77 cases in RT-PCR method; 81 in ELISA; 82 in immunocolloidal gold test; 92 in combined the three methods. In 223 with non-hepatitis C patients, false positive was found one in RT-PCR; 43 in ELISA and 48 in immunocolloidal gold test. Conclusion HCV-RNA quantitative detection has a high specificity, both ELISA and immunocolloidal gold test have a high false positivity. Employing combination of the three test methods can raise detection rate of HCV and reduce its false positive.
出处
《齐鲁医学杂志》
2013年第1期65-66,共2页
Medical Journal of Qilu
关键词
肝炎
丙型
逆转录聚合酶链反应
酶联免疫吸附测定
胶体金
hepatitis C
reverse transcriptase polymerase chain reaction
enzyme linked immunosorbent assay
gold colloid