摘要
采用大孔树脂吸附法富集纯化黄芪总皂苷,以HPLC-ELSD法测定黄芪甲苷的含量作为考察指标,筛选了树脂型号、吸附流速、洗脱溶剂、洗脱流速以及洗脱溶剂用量等工艺条件。结果表明:最佳工艺为选择D101型大孔树脂,吸附流速为2 BV.h-1,洗脱流速为4 BV.h-1,收集5 BV的70%乙醇部分,得到的黄芪总皂苷纯化效果最好,黄芪甲苷的转移率可达93.21%。
The aim of this study was to investigate the enrichment and purification methods of astragalo- sides from Radix Astragali with macroporous resins. Taking the content of astragaloside IV determined by HPLC - ELSD as index, the technological parameter including type of resin, absorption rate, elution sol- vent, rate and volume of elution were optimized. The results showed that the best condition for separation and purification of astragalosides with D101 was as follows： absorption rate of 2 BV·h-1, 5 BV of 70% ethanol as the elution solvent and the elution flow rate of 4 BV·h-1. Under the optimal condition, the ex- traction rate of astragaloside IV reached 93.21%.
出处
《中国野生植物资源》
2013年第1期18-20,28,共4页
Chinese Wild Plant Resources
基金
中央高校基本科研业务费专项基金(JKY2011079)
